MDS42: Difference between revisions
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==Construction== | ==Construction== | ||
Most of the deletions in MDS42 were constructed by lambda Red-mediated recombination of linear fragments into the genome, followed by removal of exogenous sequences by double strand break (DSB)-stimulated recombination. | |||
==Notes== | ==Notes== | ||
Revision as of 00:56, 30 January 2010
See Help:Strains for more information about strains in EcoliWiki.
Alternate Names
Sources
Chromosomal Genotype
MG1655 fhuACDB(del) endA(del) + deletion 699 additional genes, including all IS elements and cryptic prophages[1]
Plasmids
Phage
Phenotypes
Construction
Most of the deletions in MDS42 were constructed by lambda Red-mediated recombination of linear fragments into the genome, followed by removal of exogenous sequences by double strand break (DSB)-stimulated recombination.
Notes
Compared to MG1655, MDS42 has 663,316 bp deleted (14.3% of the original genome).[1] Deletion of fhuACDB confers resistance to phage T1. The endA gene was deleted to improve the integrity of plasmid DNA isolated from this strain.
References
See Help:References for how to manage references in EcoliWiki. [1]