PMID:1998686

Chang, ZY, Nygaard, P, Chinault, AC and Kellems, RE (1991) Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function.Biochemistry 30:2273-80

Abstract

The goal of the research reported here is to identify evolutionarily conserved amino acid residues associated with enzymatic deamination of adenosine. To do this, we isolated molecular clones of the Escherichia coli adenosine deaminase gene by functional complementation of adenosine deaminase deficient bacteria and deduced the amino acid sequence of the enzyme from the nucleotide sequence of the gene. Nucleotide sequence analysis revealed the presence of a 996-nucleotide open reading frame encoding a protein of 332 amino acids having a molecular weight of 36,345. The deduced amino acid sequence of the E. coli enzyme has approximately 33% identity with those of the mammalian adenosine deaminases. With conservative amino acid substitutions the overall sequence homology approaches 50%, suggesting that the structures and functions of the mammalian and bacterial enzymes are similar. Additional amino acid sequence analysis revealed specific residues that are conserved among all three adenosine deaminases and four AMP deaminases for which sequence information is currently available. In view of previously published enzymological data and the conserved amino acid residues identified in this study, we propose a model to account for the enzyme-catalyzed hydrolytic deamination of adenosine. Potential catalytic roles are assigned to the conserved His 214, Cys 262, Asp 295, and Asp 296 residues of mammalian adenosine deaminases and the corresponding conserved amino acid residues in bacterial adenosine deaminase and the eukaryotic AMP deaminases.

Links

PubMed

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
add:Gene_Product(s)		GO:0009168	purine ribonucleoside monophosphate biosynthetic process	IGI: Inferred from Genetic Interaction	EcoliWiki:guaA	P	A double mutant of guaA and add cannot grow on minimal media without supplementation with guanosine (or guanine) and DAP. Expression of a cloned add in trans from a plasmid allows the same strain to grow on minimal media containing DAP (see pp. 2275).	complete
add:Gene_Product(s)		GO:0015950	purine nucleotide interconversion	IGI: Inferred from Genetic Interaction	EcoliWiki:guaA	P	A double mutant of guaA and add cannot grow on minimal media without supplementation with guanosine (or guanine) and DAP. Expression of a cloned add in trans from a plasmid allows the same strain to grow on minimal media containing DAP (see pp. 2275).	complete
add:Gene_Product(s)		GO:0006154	adenosine catabolic process	IGI: Inferred from Genetic Interaction	EcoliWiki:guaA	P	A double mutant of guaA and add cannot grow on minimal media without supplementation with guanosine (or guanine) and DAP. Expression of a cloned add in trans from a plasmid allows the same strain to grow on minimal media containing DAP (see pp. 2275).	complete
add:Gene_Product(s)		GO:0032261	purine nucleotide salvage	IGI: Inferred from Genetic Interaction	EcoliWiki:guaA	P	A double mutant of guaA and add cannot grow on minimal media without supplementation with guanosine (or guanine) and DAP. Expression of a cloned add in trans from a plasmid allows the same strain to grow on minimal media containing DAP (see pp. 2275).	complete
add:Gene_Product(s)		GO:0043103	hypoxanthine salvage	IGI: Inferred from Genetic Interaction	EcoliWiki:guaA	P	A double mutant of guaA and add cannot grow on minimal media without supplementation with guanosine (or guanine) and DAP. Expression of a cloned add in trans from a plasmid allows the same strain to grow on minimal media containing DAP (see pp. 2275).	complete
add:Gene_Product(s)		GO:0046101	hypoxanthine biosynthetic process	IGI: Inferred from Genetic Interaction	EcoliWiki:guaA	P	A double mutant of guaA and add cannot grow on minimal media without supplementation with guanosine (or guanine) and DAP. Expression of a cloned add in trans from a plasmid allows the same strain to grow on minimal media containing DAP (see pp. 2275).	complete
edit table

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.