speE:Gene Product(s): Difference between revisions

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Revision as of 19:42, 8 October 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SpeE

Synonyms

spermidine synthase (putrescine aminopropyltransferase)[1], B0121[2][1], SpeE[2][1]

Product description

SpeE[2][3];

Component of spermidine synthase[2][3]

Spermidine synthase[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [5]

complete

GO:0008295

spermidine biosynthetic process

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0745
HAMAP:MF_00198

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0006596

polyamine biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0620

P

Seeded from EcoCyc [5]

complete

GO:0004766

spermidine synthase activity

GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

EC:2.5.1.16
HAMAP:MF_00198

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001045

F

Seeded from EcoCyc [5]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0005829

cytosol

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [5]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of spermidine synthase

could be indirect

Protein

argS

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

rpsL

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

yacL

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

evgA

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

nrdI

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

groL

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

yedD

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

cueO

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

uspG

PMID:16606699[8]

Experiment(s):EBI-1135645

Protein

rplN

PMID:16606699[8]

Experiment(s):EBI-1135645

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MAEKKQWHET LHDQFGQYFA VDNVLYHEKT DHQDLIIFEN AAFGRVMALD GVVQTTERDE
FIYHEMMTHV PLLAHGHAKH VLIIGGGDGA MLREVTRHKN VESITMVEID AGVVSFCRQY
LPNHNAGSYD DPRFKLVIDD GVNFVNQTSQ TFDVIISDCT DPIGPGESLF TSAFYEGCKR
CLNPGGIFVA QNGVCFLQQE EAIDSHRKLS HYFSDVGFYQ AAIPTYYGGI MTFAWATDND
ALRHLSTEII QARFLASGLK CRYYNPAIHT AAFALPQYLQ DALASQPS
Length

288

Mol. Wt

32.32 kDa

pI

5.5 (calculated)

Extinction coefficient

30,370 - 31,120 (calc based on 13 Y, 2 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes

Initiator Methionine

1

Removed

UniProt:P09158

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=speE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:SPERMIDINESYN-MONOMER

UniProt (EcoliWiki Page)

UniProt:P09158

PFAM (EcoliWiki Page)

PFAM:PF01564

RefSeq (EcoliWiki Page)

RefSeq:NP_414663


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  6. ↑ 6.0 6.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed

Categories

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