DH10B: Difference between revisions

Revision as of 03:20, 15 September 2012

See Help:Strains for more information about strains in EcoliWiki.

Alternate Names

The sequence of DH10B was published in 2008^[1] .

DH10B

Sources

Chromosomal Genotype

F^- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ^-
From J Jessee.^[2]

Plasmids

not specified

Phage

not specified

Phenotypes

Construction

unknown parents.

Comments

useful for generating genomic libraries containing methylated cytosine or adenine residues; useful for plasmid rescue procedures (Copied from GIBCO)

References

See Help:References for how to manage references in EcoliWiki.

↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
↑ Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed

[PMID:18245285-1] Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed

[PMID:2162051-2] Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed

[1]

[2]

@@ Line 18: / Line 18: @@
 unknown parents.
 == Comments ==
-host for pUC and other α-complementation vectors; pBR322<br />
+useful for generating genomic libraries containing methylated cytosine or adenine residues; useful for plasmid rescue procedures (Copied from GIBCO)
-useful for generating genomic libraries containing methylated cytosine or adenine residues<br />
-useful for plasmid rescue procedures<br />
-Copied from GIBCO
 == See Also ==