ispB:Gene Product(s): Difference between revisions

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Protein
Protein
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Subunits of [[:Category:Complex:octaprenyl diphosphate synthase|octaprenyl diphosphate synthase]]  
[http://www.uniprot.org/uniprot/Subuni Subuni]ts of [[:Category:Complex:octaprenyl diphosphate synthase|octaprenyl diphosphate synthase]]  
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could be indirect  
could be indirect  
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Protein
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[[rplB:Gene_Product(s)|rplB]]<br />
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|[[PMID:16606699]]<ref name=PMID:16606699/>
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Experiment(s):[http://www.ebi.ac.uk/iintact/search/do/search?searchString=EBI-1145102 EBI-1145102]<br />


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Revision as of 06:13, 6 February 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

IspB

Synonyms

octaprenyl diphosphate synthase[1], B3187[2][1], Cel[2][1], YhbD[2][1], IspB[2][1]

Product description

OPPSYN[2][3];

Component of octaprenyl diphosphate synthase[3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki. See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0004659

prenyltransferase activity

PMID:11825609[4]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005515

protein binding

PMID:16606699[6]

IPI: Inferred from Physical Interaction

UniProtKB:P60422

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0016094

polyprenol biosynthetic process

PMID:11108713[7]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0006744

ubiquinone biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of octaprenyl diphosphate synthase

could be indirect

Protein

rplB

 PMID:16606699[6]

Experiment(s):EBI-1145102

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Notes

Localization

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Compartment Description Evidence Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM IAVLAARAVG
YEGNAHVTIA ALIEFIHTAT LLHDDVVDES DMRRGKATAN AAFGNAASVL VGDFIYTRAF
QMMTSLGSLK VLEVMSEAVN VIAEGEVLQL MNVNDPDITE ENYMRVIYSK TARLFEAAAQ
CSGILAGCTP EEEKGLQDYG RYLGTAFQLI DDLLDYNADG EQLGKNVGDD LNEGKPTLPL
LHAMHHGTPE QAQMIRTAIE QGNGRHLLEP VLEAMNACGS LEWTRQRAEE EADKAIAALQ
VLPDTPWREA LIGLAHIAVQ RDR
Mol. Wt

35.22 kDa (calc) [2]

pI
Extinction coefficient

24410 - 24785 (calc based on 9 Y, 2 W, and 3 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes
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Structure

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Structures

<beststructure>ispB 562</beststructure>

Models

View models at:

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:OPPSYN-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AD57

PFAM (EcoliWiki Page)

PFAM:PF00348

RefSeq (EcoliWiki Page)

RefSeq:NP_417654

ModBase (EcoliWiki Page)

ModBase:P0AD57


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ Pan, JJ et al. (2002) Insight into the activation mechanism of Escherichia coli octaprenyl pyrophosphate synthase derived from pre-steady-state kinetic analysis. Biochim Biophys Acta 1594 64-73 PubMed
  5. ↑ 5.0 5.1 5.2 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. ↑ 6.0 6.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ Kainou, T et al. (2001) Dimer formation of octaprenyl-diphosphate synthase (IspB) is essential for chain length determination of ubiquinone. J Biol Chem 276 7876-83 PubMed


Categories

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