DH10B: Difference between revisions
Jump to navigation
Jump to search
imported>Wikientrybot Make better help links. |
|||
| Line 1: | Line 1: | ||
{{Strain Help}} | |||
== Alternate Names == | |||
The sequence of DH10B was published in 2008<ref name='PMID:18245285'/> . | The sequence of DH10B was published in 2008<ref name='PMID:18245285'/> . | ||
DH10B | DH10B | ||
==Sources | ==Sources == | ||
==Chromosomal Genotype | ==Chromosomal Genotype == | ||
F<sup>-</sup> [[mcrA]] Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup><br /> | F<sup>-</sup> [[mcrA]] Δ(''mrr''-''hsd''RMS-''mcr''BC) Φ80d''lac''ZΔM15 Δ''lac''X74 ''end''A1 ''rec''A1 ''deo''R Δ(''ara'',''leu'')7697 ''ara''D139 ''gal''U ''gal''K ''nup''G ''rps''L λ<sup>-</sup><br /> | ||
From J Jessee.<ref name='PMID:2162051'/> | From J Jessee.<ref name='PMID:2162051'/> | ||
==Plasmids | ==Plasmids == | ||
not specified | not specified | ||
==Phage | ==Phage == | ||
not specified | not specified | ||
==Phenotypes | ==Phenotypes == | ||
==Construction | ==Construction == | ||
unknown parents. | unknown parents. | ||
== Comments | == Comments == | ||
host for pUC and other α-complementation vectors; pBR322<br /> | host for pUC and other α-complementation vectors; pBR322<br /> | ||
useful for generating genomic libraries containing methylated cytosine or adenine residues<br /> | useful for generating genomic libraries containing methylated cytosine or adenine residues<br /> | ||
| Line 20: | Line 23: | ||
Copied from GIBCO | Copied from GIBCO | ||
== References | == References == | ||
{{RefHelp}}<references/> | {{RefHelp}}<references/> | ||
__NOTOC__ | __NOTOC__ | ||
[[Category:Strains]] | [[Category:Strains]] | ||
Revision as of 19:42, 25 February 2009
See Help:Strains for more iformation about strains in EcoliWiki.
Alternate Names
The sequence of DH10B was published in 2008[1] .
DH10B
Sources
Chromosomal Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
From J Jessee.[2]
Plasmids
not specified
Phage
not specified
Phenotypes
Construction
unknown parents.
Comments
host for pUC and other α-complementation vectors; pBR322
useful for generating genomic libraries containing methylated cytosine or adenine residues
useful for plasmid rescue procedures
Copied from GIBCO
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
- ↑ Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed