XL-2 Blue: Difference between revisions
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{{Strain Help}} | |||
==Sources | |||
==Alternate Names == | |||
==Sources == | |||
[http://www.stratagene.com Stratagene] | [http://www.stratagene.com Stratagene] | ||
==Chromosomal Genotype | ==Chromosomal Genotype == | ||
''end''A1 ''sup''E44 ''thi''-1 ''rec''A1 ''gyr''A96 ''rel''A1 ''lac'' | ''end''A1 ''sup''E44 ''thi''-1 ''rec''A1 ''gyr''A96 ''rel''A1 ''lac'' | ||
==Plasmids | ==Plasmids == | ||
==Phage | ==Phage == | ||
F´ ''pro''AB ''lac''IqZΔM15 Tn10 (Tetr) | F´ ''pro''AB ''lac''IqZΔM15 Tn10 (Tetr) | ||
==Phenotypes | ==Phenotypes == | ||
Tetracycline resistant | Tetracycline resistant | ||
==Construction | ==Construction == | ||
==Notes== | ==Notes== | ||
Latest revision as of 19:42, 25 February 2009
See Help:Strains for more iformation about strains in EcoliWiki.
Alternate Names
Sources
Chromosomal Genotype
endA1 supE44 thi-1 recA1 gyrA96 relA1 lac
Plasmids
Phage
F´ proAB lacIqZΔM15 Tn10 (Tetr)
Phenotypes
Tetracycline resistant
Construction
Notes
The XL-2 Blue MRF' (Minus Restriction) strain is deficient in all known restriction systems (McrA-, McrCB-, McrF-, Mrr-, HsdR-), preventing cleavage of cloned DNA that carries cytosine and/or adenine methylation, which is often present in eukaryotic DNA and cDNA.(Stratagene)
References
See Help:References for how to manage references in EcoliWiki.