XL-2 Blue
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Alternate Names
Sources
Chromosomal Genotype
endA1 supE44 thi-1 recA1 gyrA96 relA1 lac
Plasmids
Phage
F´ proAB lacIqZΔM15 Tn10 (Tetr)
Phenotypes
Tetracycline resistant
Construction
Notes
The XL-2 Blue MRF' (Minus Restriction) strain is deficient in all known restriction systems (McrA-, McrCB-, McrF-, Mrr-, HsdR-), preventing cleavage of cloned DNA that carries cytosine and/or adenine methylation, which is often present in eukaryotic DNA and cDNA.(Stratagene)
References
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