Category:Strain:MC1061: Difference between revisions
Table edited by Wouter.deschamphelaire via TableEdit |
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== Notes == | == Notes == | ||
Useful host for primary transformation. Transforms very well by the CaCl2 method (10<sup>7</sup>/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification.<br /> | |||
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability.<br /> | |||
The zeocin resistance is most probably related to the presence of a functional recA gene (recA<sup>+</sup>). | |||
==References== | ==References== | ||
Revision as of 09:42, 4 July 2016
See Help:Strains for more information about strains in EcoliWiki.
| Name |
MC1061 |
|---|---|
| Synonyms | |
| Genotype |
araD139 Del(araA-leu)7697 Del(lac)X74 galK16 galE15(GalS) lambda- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2 This is the genotype at CGSC as of Nov, 2008. Note that there are differences with the original genotype [1] |
| Phenotype |
streptomycin resistance |
| Plasmids | |
| Phage | |
| Sources | |
| Construction |
Made by Malcolm Casadaban |
| References | |
| edit table |
Notes
Useful host for primary transformation. Transforms very well by the CaCl2 method (107/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification.
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability.
The zeocin resistance is most probably related to the presence of a functional recA gene (recA+).
References
See Help:References for how to manage references in EcoliWiki.
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