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araD139 Del(araA-leu)7697 Del(lac)X74 galK16 galE15(GalS) lambda- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2
This is the genotype at CGSC as of Nov, 2008. Note that there are differences with the original genotype 
Made by Malcolm Casadaban
Useful host for primary transformation. Transforms very well by the CaCl2 method (107/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification.
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability.
The zeocin resistance is most probably related to the presence of a functional recA gene (recA+).
See Help:References for how to manage references in EcoliWiki.
- Casadaban, MJ & Cohen, SN (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138 179-207 PubMed
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