Category:Strain:BW25113: Difference between revisions
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!align=left {{table_heading_style}} |Name | !align=left {{table_heading_style}} |Name | ||
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BW25113 | |||
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!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
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!align=left {{table_heading_style}} |Genotype | !align=left {{table_heading_style}} |Genotype | ||
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F<sup>-</sup>, DE(araD-araB)567, lacZ4787(del)::rrnB-3, LAM<sup>-</sup>, rph-1, DE(rhaD-rhaB)568, hsdR514 | |||
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!align=left {{table_heading_style}} |Phenotype | !align=left {{table_heading_style}} |Phenotype | ||
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!align=left {{table_heading_style}} |Sources | !align=left {{table_heading_style}} |Sources | ||
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[http://cgsc.biology.yale.edu/Strain.php?ID=64667 Coli Genetics Stock Center] | |||
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!align=left {{table_heading_style}} |Construction | !align=left {{table_heading_style}} |Construction | ||
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see description below | |||
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!align=left {{table_heading_style}} |References | !align=left {{table_heading_style}} |References | ||
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PMID:10829079 | |||
PMID:16738554 | |||
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Revision as of 17:24, 14 June 2013
See Help:Strains for more information about strains in EcoliWiki.
| Name |
BW25113 |
|---|---|
| Synonyms | |
| Genotype |
F-, DE(araD-araB)567, lacZ4787(del)::rrnB-3, LAM-, rph-1, DE(rhaD-rhaB)568, hsdR514 |
| Phenotype | |
| Plasmids | |
| Phage | |
| Sources | |
| Construction |
see description below |
| References | |
| edit table |
Notes
BW25113 is a derivative of the F-, λ-, E. coli K-12 strain BD792 (CGSC6159)[1], which is a derivative of E. coli K-12 W1485. According to the CGSC, BD792 carries rpoS396(Am), but this mutation is not listed in the published genotype for BW25113, as according to Baba 2006 [2], a pseudoreversion rpoS (Q33) allele was transferred from MG1655 into a predecessor of BW25113.
BW25113 was used to make the Keio Collection of single-gene knockouts. [2] [1] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [1] said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1)[2].
According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
- ↑ 2.0 2.1 2.2 2.3 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed