pJH370: Difference between revisions
m →History |
m →History |
||
| Line 53: | Line 53: | ||
{{PlasmidHistory}} | {{PlasmidHistory}} | ||
pJH370 was constructed by Jim Hu in Bob Sauer's lab to study self-assembly by the GCN4 Leucine Zipper <ref name='PMID:2147779'/>. | pJH370 was constructed by Jim Hu in Bob Sauer's lab to study self-assembly by the GCN4 Leucine Zipper <ref name='PMID:2147779'/>. | ||
pJH370 was constructed from Fred Gimble's pFG157 plasmid, which expresses [[Phage lambda cI:Quickview|lambda cI]] from the lacUV5 promoter. The C-terminal domain of cI was replaced by an artificial construct encoding the GCN4 leucine zipper. | |||
==Sources== | ==Sources== | ||
Revision as of 22:04, 15 January 2013
See Help:Plasmid for more information about plasmids in EcoliWiki.
At a Glance
| Type | |
|---|---|
| Origin of Replication |
pBR322 plus f1 |
| Copy # |
medium |
| Markers |
Amp |
| Link to Sequence | |
| Features |
cloning vector contains bacteriophage lambda cI N-terminal domain fused to the GCN4 leucine zipper |
| Accession Number | |
| edit table |
Notes
History
You can help EcoliWiki by writing about the history of this plasmid. pJH370 was constructed by Jim Hu in Bob Sauer's lab to study self-assembly by the GCN4 Leucine Zipper [1].
pJH370 was constructed from Fred Gimble's pFG157 plasmid, which expresses lambda cI from the lacUV5 promoter. The C-terminal domain of cI was replaced by an artificial construct encoding the GCN4 leucine zipper.
Sources
You can help EcoliWiki by writing about the available sources of this plasmid.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.