rlmH:Gene Product(s): Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
Gribskov (talk | contribs)
touch
 
(43 intermediate revisions by 3 users not shown)
Line 10: Line 10:
-->{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
-->{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
<noinclude>{{TabsTop}}
{{Tab|Quickview|ybeA}}{{Tab|Gene|ybeA}}{{Tab On|Gene Product(s)|ybeA}}{{Tab|Expression|ybeA}}{{Tab|Evolution|ybeA}}{{Tab|On One Page|ybeA}}{{TabsBottom}}
{{Tab|Quickview|rlmH}}{{Tab|Gene|rlmH}}{{Tab On|Gene Product(s)|rlmH}}{{Tab|Expression|rlmH}}{{Tab|Evolution|rlmH}}{{Tab|On One Page|rlmH}}{{TabsBottom}}
{|
{|
|-  
|-  
Line 18: Line 18:
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Gene Product Resources|Resources]]
Line 33: Line 34:
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_nomenclature_help}}
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  
{|   id="S46aa3ad7c752a" class=" tableEdit Product_nomenclature_table"


|-
|-
!align=left {{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
||
||
YbeA product, rRNA large subunit methyltransferase
rlmH, rRNA large subunit methyltransferase
|-
|-
!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
conserved protein product<ref name='LIB:Riley_2006'/>, B0636 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YbeA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''conserved protein<ref name='LIB:Riley_2006'/>, B0636<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YbeA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK0629, JW0631, ybeA, b0636''
|-
|-
!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
||
||
conserved protein<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>; Component of ; [[:Category:Complex:conserved protein|conserved protein]]<ref name='LIB:EcoCyc11.1'/>.  Sequence similarity indicates ybeA is an S-adenosylmethionine-dependent (SPOUT) methyltransferase <ref name="PMID:18755835"/>.  Inactivation of the ybeA gene (Keio collection knockout <ref name='PMID:16738554'/>) leads to loss of methylation at nucleotide Psi1915 in 23S rRNA <ref name="PMID:18755835"/>.  Computational docking suggests that a YbeA methyltransferase homodimer fits comfortably into the ribosomal A site without encroaching into the P site <ref name="PMID:18755835"/>.
conserved protein<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>; Component of [[:Category:Complex:conserved protein|conserved protein]]<ref name='LIB:EcoCyc11.1'/>.  Sequence similarity indicates rlmH is an S-adenosylmethionine-dependent (SPOUT) methyltransferase <ref name="PMID:18755835"/>.  Inactivation of the rlmH gene (Keio collection knockout <ref name='PMID:16738554'/>) leads to loss of methylation at nucleotide Psi1915 in 23S rRNA <ref name="PMID:18755835"/>.  Computational docking suggests that a YbeA methyltransferase homodimer fits comfortably into the ribosomal A site without encroaching into the P site <ref name="PMID:18755835"/>.


Purta <em>et al.</em> suggest that a more suitable designation
Purta <em>et al.</em> suggest that a more suitable designation
Line 58: Line 59:
Press, Washington, DC.'/>, and Andersen and Douthwaite, 2006 <ref name='PMID:16678201'/>.
Press, Washington, DC.'/>, and Andersen and Douthwaite, 2006 <ref name='PMID:16678201'/>.


Putative methyltransferase, function unknown<ref name='LIB:EcoGene'/>
|-
|-
!align=left {{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
||
||
*


|-class='sortbottom'
 
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.S46aa3ad7c752a&page=20997&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.S46aa3ad7c752a&page=20997&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table]</span> ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.S46aa3ad7c752a-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.S46aa3ad7c752a-->
{{Product_nomenclature_help}}
 
<protect><!--Nomenclature_notes--></protect>
<protect><!--Nomenclature_notes--></protect>
===Notes===
===Notes===
Line 75: Line 77:
{{BacktoTop}}
{{BacktoTop}}
<protect>
<protect>
<big>'''Gene Ontology'''</big><br />{{Product_GO_help}}<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.O46aa3ad928a57-->
<big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.20997.O46aa3ad928a57-->
<!--
<!--
******************************************************************************************
******************************************************************************************
Line 81: Line 83:
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_GO_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{|   id="O46aa3ad928a57" class=" tableEdit GO_table_product dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
 
|
GO:0005737
|
cytoplasm
|
GOA:hamap<br />GO_REF:0000020
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00658
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005737
|
cytoplasm
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR003742
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005737
|
cytoplasm
|
GOA:spkw<br />SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0963
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005737
|
cytoplasm
|
GO_REF:0000023
|
IEA: Inferred from Electronic Annotation
|
SP_SL:SL-0086
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005737
|
cytoplasm
|
PMID:18755835
|
IC: Inferred by Curator
|
GO:0070038
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0005515_!_protein_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005515_!_protein_binding GO:0005515]
GO:0031167
|
rRNA methylation
|
PMID:18755836
|
IDA: Inferred from Direct Assay
|
|
protein_binding
 
|
|
[[PMID:15690043]]<ref name=PMID:15690043/><br />
P
|
|
IPI: Inferred from Physical Interaction
Seeded from [[EcoCyc]] (v14.0)
|
|
UniProtKB:P0A7V0
complete
|-
|
 
|
GO:0042803
|
protein homodimerization activity
|
PMID:18755835
|
NAS: Non-traceable Author Statement
|
 
|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0043022
|
ribosome binding
|
PMID:18755835
|
NAS: Non-traceable Author Statement
|
 
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0070038
|
rRNA (pseudouridine-N3-)-methyltransferase activity
|
GOA:hamap<br />GO_REF:0000020
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00658
|
F
|
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0070475
|
rRNA base methylation
|
PMID:18755835
|
IMP: Inferred from Mutant Phenotype
|
 
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0070475
|
rRNA base methylation
|
PMID:18755836
|
IMP: Inferred from Mutant Phenotype
|
 
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete


|-class='sortbottom'
|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.O46aa3ad928a57&page=20997&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.O46aa3ad928a57&page=20997&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table]</span> || || || || || || || ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.O46aa3ad928a57-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.O46aa3ad928a57-->
Line 117: Line 309:
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_interactions_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="N46aa3ad99a676"  class=" tableEdit Product_interactions_table dataTable"   
|- {{table heading style}}
|- {{table heading style}}
!|Partner Type!!Partner!!Notes
!|Partner Type!!Partner!!Notes!!References!!Evidence
|-  
|-  
|
|
Line 127: Line 319:
Subunits of [[:Category:Complex:conserved protein|conserved protein]]  
Subunits of [[:Category:Complex:conserved protein|conserved protein]]  
|
|
could be indirect   
could be indirect  
|
 
|
   
|-
|
Protein
|
rpsB<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-887739<br />
|-
|
Protein
|
groL<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1137503<br />
|-
|
Protein
|
gfcD<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1137503<br />
|-  
|-  
|
|
Line 134: Line 363:
Homodimer
Homodimer
|
|
Docking simulation and sequence similarity <ref name='PMID:18755835'/>  
Docking simulation and sequence similarity <ref name='PMID:18755835'/>
|
 
|


|-class='sortbottom'
|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.N46aa3ad99a676&page=20997&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.N46aa3ad99a676&page=20997&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table]</span> || || || ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.N46aa3ad99a676-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.N46aa3ad99a676-->
{{Product_interactions_help}}
 
<!--Function_notes--></protect>
<!--Function_notes--></protect>
===Notes===
===Notes===
Line 149: Line 382:
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_localization_help}}
{|class = 'sortable' {{Prettytable}} id='105031'
{|  id="S46a7cc6885728"  class=" tableEdit Product_localization_table" 
|- {{table heading style}}
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
|-class='sortbottom'
 
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.S46a7cc6885728&page=20997&pagename={{FULLPAGENAMEE}} edit table] || || ||
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.S46a7cc6885728&page=20997&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table]</span> || || || ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.S46a7cc6885728-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.S46a7cc6885728-->
{{Product_localization_help}}
 
<protect><!--Localization_notes--></protect>
<protect><!--Localization_notes--></protect>


Line 173: Line 407:
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_physical_properties_help}}
{|{{Prettytable}}
{|   id="M46aa3adbb4ca2"  class=" tableEdit Product_phys_prop_table" 
 
|-
!align=left {{table heading style}} |Name
||
 
|-
|-
!{{table heading style}} |Sequence
!align=left {{table heading style}} |Sequence
|
||
[http://biocyc.org/ECOLI/sequence-aa?object=EG11254-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=EG11254-MONOMER at EcoCyc]  
<pre>MKLQLVAVGT KMPDWVQTGF TEYLRRFPKD MPFELIEIPA GKRGKNADIK RILDKEGEQM
<pre>MKLQLVAVGT KMPDWVQTGF TEYLRRFPKD MPFELIEIPA GKRGKNADIK RILDKEGEQM
Line 186: Line 425:
SLSALTLPHP LVRVLVAESL YRAWSITTNH PYHRE</pre>  
SLSALTLPHP LVRVLVAESL YRAWSITTNH PYHRE</pre>  
|-
|-
!{{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Length
|
||
17.34 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
155
|-
|-
!{{table heading style}} |pI
!align=left {{table heading style}} |Mol. Wt
|
||
17.341 kDa
|-
|-
!{{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |pI
|
||
31970 - 32095 (calc based on 3 Y, 5 W, and 1 C residues)  
8.9 (calculated)
|-
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.M46aa3adbb4ca2&page=20997&pagename={{FULLPAGENAMEE}} edit table]
!align=left {{table heading style}} |Extinction coefficient
||
31,970 - 32,095 (calc based on 3 Y, 5 W, and 1 C residues)
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.M46aa3adbb4ca2&page=20997&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table]</span> ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.M46aa3adbb4ca2-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.M46aa3adbb4ca2-->
{{Product_physical_properties_help}}


<big>'''Domains/Motifs/Modification Sites'''</big>
 
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.P46aa3adc1b46c-->
<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table>
<tr align="left" valign="top">
<td><!--box uid=421aa90e079fa326b6494f812ad13e79.20997.P46aa3adc1b46c-->
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_domains_motifs_help}}
{|class = 'sortable' {{Prettytable}} id='156034'
{|  id="P46aa3adc1b46c"  class=" tableEdit Product_motif_table dataTable" 
|- {{table heading style}}
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-class='sortbottom'
|-
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.P46aa3adc1b46c&page=20997&pagename={{FULLPAGENAMEE}} edit table] || || ||
|
Domain
|
1..153
|
[http://pfam.janelia.org/family/PF02590 PF02590  Predicted SPOUT methyltransferase]
|
 
|
PMID:19920124
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.P46aa3adc1b46c&page=20997&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table]</span> || || || ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.P46aa3adc1b46c-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.P46aa3adc1b46c--></td>
{{Product_domains_motifs_help}}
<td>
<!-- motifs column 2-->
<motif_map/>
</td>
</tr>
</table>


<span id="Structure"></span><big>'''Structure'''</big>
<table><tr valign='top'><td>
<span id='Structure'></span><big>'''Structure'''</big><br />
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.J46aa3adc65ccb-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.J46aa3adc65ccb-->
<!--
<!--
Line 227: Line 491:
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_structure_help}}
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  
{|   id="J46aa3adc65ccb" class=" tableEdit Product_structure_table"


|-
|-
!align=left {{table heading style}} |Structures
!align=left {{table heading style}} |Structures
||
||
<beststructure>ybeA
<beststructure>
562</beststructure>
gene=ybeA
 
taxon=562,83333
</beststructure>
|-
|-
!align=left {{table heading style}} |Models
!align=left {{table heading style}} |Models
||
||
View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=ybeA&sub=keywordSearc EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P0A8I8 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=ybeA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0A8I8 PSI-PRED (P0A8I8)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0A8I8 Genomes TO Protein (P0A8I8)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


|-class='sortbottom'
|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.J46aa3adc65ccb&page=20997&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.J46aa3adc65ccb&page=20997&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table]</span> ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.J46aa3adc65ccb-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.J46aa3adc65ccb-->
{{Product_structure_help}}
 
<!--Structure_notes--></protect>
</td><td></protect>
==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
<protect><!--          ***** PLEASE DO NOT EDIT BELOW THIS LINE *****        -->
</td><tr><table><!--Structure_notes--></protect>
===Notes===
===Notes===


Line 261: Line 544:
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Product_resources_help}}
{|class = 'sortable' {{Prettytable}} id='156036'
{|  id="L46aa3add24865"  class=" tableEdit Product_resource_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
!|Resource type!!Source!!Notes/Reference
|-class='sortbottom'
 
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.L46aa3add24865&page=20997&pagename={{FULLPAGENAMEE}} edit table] || ||
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.L46aa3add24865&page=20997&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table]</span> || ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.L46aa3add24865-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.L46aa3add24865-->
{{Product_resources_help}}
 
<protect><!--Resources_notes--></protect>
<protect><!--Resources_notes--></protect>
===Notes===
===Notes===
Line 282: Line 566:
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->{{Gene_accessions_help}}
{|class = 'sortable' {{Prettytable}} id='156037'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X46aa3add6fd7f"  class=" tableEdit Gene_accessions_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11254-MONOMER EcoCyc:EG11254-MONOMER]
[http://www.ncbi.nlm.nih.gov/protein/16128619 GI:16128619]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
|
[[uniprot:P0A8I8|UniProt:P0A8I8]] 
[http://www.ncbi.nlm.nih.gov/protein/NP_415169.1 GeneID:945239]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
|
[http://pfam.sanger.ac.uk/family?acc=PF02590 PFAM:PF02590]
[http://www.uniprot.org/uniprot/P0A8I8 UniProtKB/Swiss-Prot:P0A8I8]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_415169 RefSeq:NP_415169]
[http://www.ncbi.nlm.nih.gov/gene?term=945239 GeneID:945239]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A8I8 ModBase:P0A8I8] 
RegulonDB:
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://www.rcsb.org/pdb PDB] ([[PDB|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NS5 PDB:1NS5] 
EchoBASE:
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-class='sortbottom'
 
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.X46aa3add6fd7f&page=20997&pagename={{FULLPAGENAMEE}} edit table] || ||
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.X46aa3add6fd7f&page=20997&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table]</span> || ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.X46aa3add6fd7f-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.X46aa3add6fd7f-->
{{Product_accessions_help}}
 
<protect><!--Accessions_notes--></protect>
<protect><!--Accessions_notes--></protect>
===Notes===
===Notes===
Line 345: Line 630:
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->
{|class = 'sortable' {{Prettytable}} id='105034'
{|   id="H46a7cc6ebbe77"  class=" tableEdit Links_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Name!!URL!!Comments
!|Name!!URL!!Comments
|-class='sortbottom'
 
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.H46a7cc6ebbe77&page=20997&pagename={{FULLPAGENAMEE}} edit table] || ||
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20997.H46a7cc6ebbe77&page=20997&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table]</span> || ||
|}
|}
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.H46a7cc6ebbe77-->
<!--box uid=421aa90e079fa326b6494f812ad13e79.20997.H46a7cc6ebbe77-->
{{Links_help}}
 




Line 364: Line 650:
{{BacktoTop}}
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</noinclude>
===Categories===
===Categories===
{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:Complex:conserved protein]][[Category:Products with structures in PDB]][[Category:Complex:23S rRNA methyltransferase]][[Category:Genes in the Keio knockout collection]]
[[Category:Complex:conserved protein]][[Category:Products with structures in PDB]][[Category:Complex:23S rRNA methyltransferase]][[Category:Genes in the Keio knockout collection]]
[[Category:Pfam:PF02590_!_Predicted SPOUT methyltransferase]]

Latest revision as of 16:47, 5 October 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

rlmH, rRNA large subunit methyltransferase

Synonyms

conserved protein[1], B0636[2][1], YbeA[2][1] , ECK0629, JW0631, ybeA, b0636

Product description

conserved protein[2][3]; Component of conserved protein[3]. Sequence similarity indicates rlmH is an S-adenosylmethionine-dependent (SPOUT) methyltransferase [4]. Inactivation of the rlmH gene (Keio collection knockout [5]) leads to loss of methylation at nucleotide Psi1915 in 23S rRNA [4]. Computational docking suggests that a YbeA methyltransferase homodimer fits comfortably into the ribosomal A site without encroaching into the P site [4].

Purta et al. suggest that a more suitable designation for the SPOUT-superfamily member YbeA would be the rRNA large subunit methyltransferase RlmH, consistent with the terminology used for the other rRNA methyltransferase characterized in E. coli (see also Ofengand and Del Campo, 2004 [6][7], and Andersen and Douthwaite, 2006 [8].

Putative methyltransferase, function unknown[9]

EC number (for enzymes)



Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00658

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003742

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:18755835[4]

IC: Inferred by Curator

GO:0070038

C

Seeded from EcoCyc (v14.0)

complete

GO:0031167

rRNA methylation

PMID:18755836[10]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0042803

protein homodimerization activity

PMID:18755835[4]

NAS: Non-traceable Author Statement

F

Seeded from EcoCyc (v14.0)

complete

GO:0043022

ribosome binding

PMID:18755835[4]

NAS: Non-traceable Author Statement

F

Seeded from EcoCyc (v14.0)

complete

GO:0070038

rRNA (pseudouridine-N3-)-methyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00658

F

Seeded from EcoCyc (v14.0)

complete

GO:0070475

rRNA base methylation

PMID:18755835[4]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0070475

rRNA base methylation

PMID:18755836[10]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of conserved protein

could be indirect

Protein

rpsB

PMID:15690043[11]

Experiment(s):EBI-887739

Protein

groL

PMID:16606699[12]

Experiment(s):EBI-1137503

Protein

gfcD

PMID:16606699[12]

Experiment(s):EBI-1137503

Protein

Homodimer

Docking simulation and sequence similarity [4]



Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes


Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKLQLVAVGT KMPDWVQTGF TEYLRRFPKD MPFELIEIPA GKRGKNADIK RILDKEGEQM
LAAAGKNRIV TLDIPGKPWD TPQLAAELER WKLDGRDVSL LIGGPEGLSP ACKAAAEQSW
SLSALTLPHP LVRVLVAESL YRAWSITTNH PYHRE
Length

155

Mol. Wt

17.341 kDa

pI

8.9 (calculated)

Extinction coefficient

31,970 - 32,095 (calc based on 3 Y, 5 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..153

PF02590 Predicted SPOUT methyltransferase

PMID:19920124[13]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ybeA taxon=562,83333 </beststructure>

Models

View models at:

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128619

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945239

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A8I8

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945239

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655


Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 Purta, E et al. (2008) YbeA is the m3Psi methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA. RNA 14 2234-44 PubMed
  5. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. ↑ Cite error: Invalid <ref> tag; no text was provided for refs named LIB:Neidhardt et al. 1996
  7. ↑ Cite error: Invalid <ref> tag; no text was provided for refs named Ofengand, J. and Del Campo, M 2004. Modified nucleotides of Escherichia coli ribosomal RNA. In EcoSal—Escherichia coli and Salmonella: Cellular and molecular biology (ed. R. Curtiss). ASM Press, Washington, DC.
  8. ↑ Andersen, NM & Douthwaite, S (2006) YebU is a m5C methyltransferase specific for 16 S rRNA nucleotide 1407. J. Mol. Biol. 359 777-86 PubMed
  9. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  10. ↑ 10.0 10.1 Ero, R et al. (2008) Identification of pseudouridine methyltransferase in Escherichia coli. RNA 14 2223-33 PubMed
  11. ↑ Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  12. ↑ 12.0 12.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  13. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

[back to top]