MDS42: Difference between revisions
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==Sources== | ==Sources== | ||
[http://scarabgenomics.com | [http://www.scarabgenomics.com Scarab Genomics] | ||
==Chromosomal Genotype== | ==Chromosomal Genotype== | ||
MG1655 fhuACDB(del) endA(del) + deletion 699 additional genes, including all IS elements and cryptic prophages<ref name='PMID:16645050'/> | MG1655 fhuACDB(del) endA(del) + deletion of 699 additional genes, including all IS elements and cryptic prophages<ref name='PMID:16645050'/> | ||
==Plasmids== | ==Plasmids== | ||
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==Construction== | ==Construction== | ||
Most of the deletions in MDS42 were constructed by lambda Red-mediated recombination of linear fragments into the genome, followed by removal of exogenous sequences by double strand break (DSB)-stimulated recombination. | Most of the deletions in MDS42 were constructed by lambda Red-mediated recombination of linear fragments into the genome, followed by removal of exogenous sequences by double strand break (DSB)-stimulated recombination.<ref name='PMID:16645050'/> The individual deletions are described in the [http://www.sciencemag.org/cgi/data/1126439/DC1/1 Supporting Online Material]. | ||
==Notes== | ==Notes== | ||
Latest revision as of 23:21, 21 February 2017
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Alternate Names
Sources
Chromosomal Genotype
MG1655 fhuACDB(del) endA(del) + deletion of 699 additional genes, including all IS elements and cryptic prophages[1]
Plasmids
Phage
Phenotypes
Construction
Most of the deletions in MDS42 were constructed by lambda Red-mediated recombination of linear fragments into the genome, followed by removal of exogenous sequences by double strand break (DSB)-stimulated recombination.[1] The individual deletions are described in the Supporting Online Material.
Notes
Compared to MG1655, MDS42 has 663,316 bp deleted (14.3% of the original genome).[1] Deletion of fhuACDB confers resistance to phage T1. The endA gene was deleted to improve the integrity of plasmid DNA isolated from this strain.
References
See Help:References for how to manage references in EcoliWiki. [1]