rffG:Gene Product(s): Difference between revisions
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|{{Sub}}[[#Localization |Localization]] | |{{Sub}}[[#Localization |Localization]] | ||
|{{Sub}}[[#Sequence|Sequence]] | |{{Sub}}[[#Sequence|Sequence]] | ||
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]] | |||
|{{Sub}}[[#Structure|Structure]] | |{{Sub}}[[#Structure|Structure]] | ||
|{{Sub}}[[#Gene Product Resources|Resources]] | |{{Sub}}[[#Gene Product Resources|Resources]] | ||
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{| id="O46ab03c8a13cb" class=" tableEdit Product_nomenclature_table" | |||
|- | |- | ||
!{{table heading style}} |Standard name | !align=left {{table heading style}} |Standard name | ||
| | || | ||
RffG | RffG | ||
|- | |- | ||
!{{table heading style}} |Synonyms | !align=left {{table heading style}} |Synonyms | ||
| | || | ||
dTDP-glucose 4,6-dehydratase | ''dTDP-glucose 4,6-dehydratase<ref name='LIB:Riley_2006'/>, B3788<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Rff<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, NfrC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, RffG<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK3780, JW5598, rff, b3788'' | ||
|- | |- | ||
!{{table heading style}} |Product description | !align=left {{table heading style}} |Product description | ||
| | || | ||
dTDP-glucose 4,6-dehydratase 2<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/> | dTDP-glucose 4,6-dehydratase 2<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/> | ||
dTDP-glucose dehydratase; needed for ECA (enterobacterial common antigen) synthesis<ref name='LIB:EcoGene'/> | |||
|- | |- | ||
!{{table heading style}} |EC number (for enzymes) | !align=left {{table heading style}} |EC number (for enzymes) | ||
| | || | ||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.46 4.2.1.46]<ref name='LIB:Riley_2006'/> | |||
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<protect><!--Nomenclature_notes--></protect> | <protect><!--Nomenclature_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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{{BacktoTop}} | {{BacktoTop}} | ||
<protect> | <protect> | ||
<big>'''Gene Ontology'''</big><br /> | <big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.28179.X46ab03c9c1a4a--> | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="X46ab03c9c1a4a" class=" tableEdit GO_table_product dataTable" | ||
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!|Qualifier!!GO ID!!GO term name!!Reference | !|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
|- | |- | ||
| | | | ||
| | | | ||
GO:0005737 | |||
| | | | ||
cytoplasm | |||
| | | | ||
| | | | ||
| | | | ||
| | | | ||
C | |||
| | | | ||
Seeded from | Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>. | ||
| | | | ||
Missing: evidence, reference | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0003824 | |||
| | | | ||
catalytic activity | |||
| | | | ||
GO_REF:0000002 | GOA:interpro<br />GO_REF:0000002 | ||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro: | InterPro:IPR001509 | ||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 116: | Line 120: | ||
| | | | ||
GO:0003824 | |||
| | | | ||
catalytic activity | |||
| | | | ||
GO_REF: | GOA:interpro<br />GO_REF:0000002 | ||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR016040 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
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| | | | ||
GO:0005488 | |||
| | | | ||
binding | |||
| | | | ||
GO_REF: | GOA:interpro<br />GO_REF:0000002 | ||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR016040 | |||
| | | | ||
F | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 154: | Line 158: | ||
| | | | ||
GO:0008152 | |||
| | | | ||
metabolic process | |||
| | | | ||
GO_REF:0000002 | GOA:interpro<br />GO_REF:0000002 | ||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro: | InterPro:IPR016040 | ||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
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| | | | ||
GO:0008460 | |||
| | | | ||
dTDP-glucose 4,6-dehydratase activity | |||
| | | | ||
GO_REF: | GOA:interpro<br />GO_REF:0000002 | ||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR005888 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
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| | | | ||
GO:0008460 | |||
| | | | ||
dTDP-glucose 4,6-dehydratase activity | |||
| | | | ||
GO_REF: | GOA:spec<br />GO_REF:0000003 | ||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
EC:4.2.1.46 | |||
| | | | ||
F | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
|- | |- | ||
| | | | ||
| | | | ||
GO:0009103 | |||
| | | | ||
lipopolysaccharide biosynthetic process | |||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
SP_KW:KW-0448 | |||
| | | | ||
P | |||
| | | | ||
Seeded from | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0009225 | |||
| | | | ||
nucleotide-sugar metabolic process | |||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
InterPro:IPR005888 | |||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0016829 | |||
| | | | ||
lyase activity | |||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
SP_KW:KW-0456 | |||
| | | | ||
F | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0044237 | |||
| | | | ||
cellular metabolic process | |||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
InterPro:IPR001509 | |||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0050662 | |||
| | | | ||
coenzyme binding | coenzyme binding | ||
| | | | ||
GO_REF:0000002 | GOA:interpro<br />GO_REF:0000002 | ||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
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F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="H46ab03e85d8fd" class=" tableEdit Product_interactions_table dataTable" | ||
|- {{table heading style}} | |- {{table heading style}} | ||
!Partner Type!!Partner!!Notes | !|Partner Type!!Partner!!Notes!!References!!Evidence | ||
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===Notes=== | ===Notes=== | ||
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!Compartment!!Description!!Evidence!!Notes | !|Compartment!!Description!!Evidence!!Reference/Source!!Notes | ||
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{|{{ | {| id="N46ab03ea5c0fc" class=" tableEdit Product_phys_prop_table" | ||
|- | |||
!align=left {{table heading style}} |Name | |||
|| | |||
|- | |- | ||
!{{table heading style}} |Sequence | !align=left {{table heading style}} |Sequence | ||
| | || | ||
[http://biocyc.org/ECOLI/sequence-aa?object=DTDPGLUCDEHYDRAT2-MONOMER at EcoCyc] | [http://biocyc.org/ECOLI/sequence-aa?object=DTDPGLUCDEHYDRAT2-MONOMER at EcoCyc] | ||
<pre>MRKILITGGA GFIGSALVRY IINETSDAVV VVDKLTYAGN LMSLAPVAQS ERFAFEKVDI | <pre>MRKILITGGA GFIGSALVRY IINETSDAVV VVDKLTYAGN LMSLAPVAQS ERFAFEKVDI | ||
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YAIDASKIAR ELGWLPQETF ESGMRKTVQW YLANESWWKQ VQDGSYQGER LGLKG</pre> | YAIDASKIAR ELGWLPQETF ESGMRKTVQW YLANESWWKQ VQDGSYQGER LGLKG</pre> | ||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Length | ||
| | || | ||
355 | |||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Mol. Wt | ||
| | || | ||
39.754 kDa | |||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |pI | ||
| | || | ||
5.9 (calculated) | |||
|- | |- | ||
| | !align=left {{table heading style}} |Extinction coefficient | ||
|| | |||
65,320 - 65,945 (calc based on 18 Y, 7 W, and 5 C residues) | |||
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<big>'''Domains/Motifs/Modification Sites'''</big> | |||
<!--box uid=421aa90e079fa326b6494f812ad13e79.28179.B46ab03ead3e09--> | <span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table> | ||
<tr align="left" valign="top"> | |||
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!Type!!Residues!!Description!!Notes | !|Type!!Residues!!Description!!Notes!!References | ||
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Domain | |||
| | |||
4..252 | |||
| | |||
[http://pfam.janelia.org/family/PF01370 PF01370 NAD dependent epimerase/dehydratase family] | |||
| | |||
| | |||
PMID:19920124 | |||
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<!-- motifs column 2--> | |||
<motif_map/> | |||
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<span id= | <table><tr valign='top'><td> | ||
<span id='Structure'></span><big>'''Structure'''</big><br /> | |||
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|-class= | !align=left {{table heading style}} |Structures | ||
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<beststructure> | |||
gene=rffG | |||
taxon=562,83333 | |||
</beststructure> | |||
|- | |||
!align=left {{table heading style}} |Models | |||
|| | |||
View models at: | |||
* [http://www.proteinmodelportal.org/query/uniprot/P27830 Protein Model Portal] | |||
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P27830 PSI-PRED (P27830)] | |||
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P27830 Genomes TO Protein (P27830)] (GTOP) | |||
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<!--Structure_notes--></protect> | </td><td></protect> | ||
==== Structure figures ==== | |||
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===Notes=== | ===Notes=== | ||
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!Resource type!!Source!!Notes/Reference | !|Resource type!!Source!!Notes/Reference | ||
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===Notes=== | ===Notes=== | ||
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!Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/49176411 GI:49176411] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
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[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
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[http://www.ncbi.nlm.nih.gov/protein/YP_026255.1 GeneID:948300] | |||
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Escherichia coli str. K-12 substr. MG1655 | |||
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[[ASAP]] | |||
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ASAP:ABE-0012376 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | |||
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[http://www.uniprot.org/uniprot/P27830 UniProtKB/Swiss-Prot:P27830] | |||
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Escherichia coli str. K-12 substr. MG1655 | |||
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[[EcoCyc]] | |||
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EcoCyc:EG11453 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EcoGene]] | |||
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EcoGene:EG11453 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]]) | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=948300 GeneID:948300] | ||
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Escherichia coli str. K-12 substr. MG1655 | |||
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[[RegulonDB]] | |||
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RegulonDB:ECK120001416 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EchoBASE]] | |||
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EchoBASE:EB1422 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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===Notes=== | ===Notes=== | ||
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===Categories=== | ===Categories=== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
[[Category:Proteins]] | [[Category:Proteins]] | ||
Latest revision as of 21:08, 17 February 2015
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
RffG |
|---|---|
| Synonyms |
dTDP-glucose 4,6-dehydratase[1], B3788[2][1], Rff[2][1], NfrC[2][1], RffG[2][1] , ECK3780, JW5598, rff, b3788 |
| Product description |
dTDP-glucose 4,6-dehydratase 2[2][3] dTDP-glucose dehydratase; needed for ECA (enterobacterial common antigen) synthesis[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005737 |
cytoplasm |
C |
Seeded from Riley et al 2006 [1]. |
Missing: evidence, reference | ||||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005488 |
binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008152 |
metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008460 |
dTDP-glucose 4,6-dehydratase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008460 |
dTDP-glucose 4,6-dehydratase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009103 |
lipopolysaccharide biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009225 |
nucleotide-sugar metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016829 |
lyase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0044237 |
cellular metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0050662 |
coenzyme binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MRKILITGGA GFIGSALVRY IINETSDAVV VVDKLTYAGN LMSLAPVAQS ERFAFEKVDI CDRAELARVF TEHQPDCVMH LAAESHVDRS IDGPAAFIET NIVGTYTLLE AARAYWNALT EDKKSAFRFH HISTDEVYGD LHSTDDFFTE TTPYAPSSPY SASKASSDHL VRAWLRTYGL PTLITNCSNN YGPYHFPEKL IPLMILNALA GKSLPVYGNG QQIRDWLYVE DHARALYCVA TTGKVGETYN IGGHNERKNL DVVETICELL EELAPNKPHG VAHYRDLITF VADRPGHDLR YAIDASKIAR ELGWLPQETF ESGMRKTVQW YLANESWWKQ VQDGSYQGER LGLKG |
| Length |
355 |
| Mol. Wt |
39.754 kDa |
| pI |
5.9 (calculated) |
| Extinction coefficient |
65,320 - 65,945 (calc based on 18 Y, 7 W, and 5 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0005737 ! cytoplasm
- GO:0003824 ! catalytic activity
- GO:0005488 ! binding
- GO:0008152 ! metabolic process
- GO:0008460 ! dTDP-glucose 4,6-dehydratase activity
- GO:0009103 ! lipopolysaccharide biosynthetic process
- GO:0009225 ! nucleotide-sugar metabolic process
- GO:0016829 ! lyase activity
- GO:0044237 ! cellular metabolic process
- GO:0050662 ! coenzyme binding
- Proteins