gcvH:Gene Product(s): Difference between revisions

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{|   id="S46aac5aebfc27"  class=" tableEdit Product_nomenclature_table"   


|-
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!align=left |Standard name
!align=left {{table heading style}} |Standard name
||
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GcvH  
GcvH  
|-
|-
!align=left |Synonyms
!align=left {{table heading style}} |Synonyms
||
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glycine cleavage complex lipoylprotein <ref name='LIB:Riley_2006'/>, Gcv <ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, B2904 <ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''glycine cleavage complex lipoylprotein <ref name='LIB:Riley_2006'/>, Gcv <ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, B2904 <ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK2899, JW2872, b2904''
|-
|-
!align=left |Product description
!align=left {{table heading style}} |Product description
||
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H-protein-(dihydrolipoyl)lysine<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>, H-protein-<i>S</i>-(aminomethyldihydrolipoyl)lysine<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
H-protein-(dihydrolipoyl)lysine<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>, H-protein-<i>S</i>-(aminomethyldihydrolipoyl)lysine<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
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Glycine cleavage, carrier of aminomethyl group<ref name='LIB:EcoGene'/>
Glycine cleavage, carrier of aminomethyl group<ref name='LIB:EcoGene'/>
|-
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!align=left |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
||
||


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{|   id="S46aac5afd0c40"  class=" tableEdit GO_table_product dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="S46aac5afd0c40"  class=" tableEdit GO_table_product dataTable"   
|-
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|


|
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GO:0005960
GO:0019464
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|
glycine cleavage complex
glycine decarboxylation via glycine cleavage system
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GO_REF:0000002
PMID:1802033
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|
IEA: Inferred from Electronic Annotation
ISS: Inferred from Sequence or Structural Similarity
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InterPro:IPR002930
UniProtKB:P11183
|
|
C
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
 
|
|
complete  
complete  
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|
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GO:0006546
GO:0005739
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glycine catabolic process
mitochondrion
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GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
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IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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InterPro:IPR002930
InterPro:IPR002930
|
|
P
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
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complete  
complete  
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|
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GO:0019464
GO:0031405
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|
glycine decarboxylation via glycine cleavage system
lipoic acid binding
|
|
GO_REF:0000020
PMID:1802033
|
|
IEA: Inferred from Electronic Annotation
ISS: Inferred from Sequence or Structural Similarity
|
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HAMAP:MF_00272
UniProtKB:P11183
|
|
P
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
 
|
|
complete  
complete  
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|
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GO:0031405
GO:0005960
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|
lipoic acid binding
glycine cleavage complex
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GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
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|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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SP_KW:KW-0450
InterPro:IPR002930
|
|
F
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
|
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GO:0009063
GO:0006546
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amino acid catabolic process  
glycine catabolic process
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GOA:interpro<br />GO_REF:0000002
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|
IEA: Inferred from Electronic Annotation
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InterPro:IPR002930
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|
P  
P
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-
|
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GO:0009257
|
10-formyltetrahydrofolate biosynthetic process
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|
|
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P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing 
|-  
|-  
|
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glycine decarboxylation via glycine cleavage system
glycine decarboxylation via glycine cleavage system
|
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PMID:1802033
GOA:hamap<br />GO_REF:0000020
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|
ISS: Inferred from Sequence or Structural Similarity
IEA: Inferred from Electronic Annotation
|
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UniProtKB:P11183
HAMAP:MF_00272
|
|
P
P
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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lipoic acid binding
lipoic acid binding
|
|
PMID:1802033
GOA:spkw<br />SP_KW:GO_REF:0000004
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|
ISS: Inferred from Sequence or Structural Similarity
IEA: Inferred from Electronic Annotation
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UniProtKB:P11183
SP_KW:KW-0450
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F
F
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
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!|Compartment!!Description!!Evidence!!Source!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes


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!align=left {{table heading style}} |Name
||


|-
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!align=left |Sequence
!align=left {{table heading style}} |Sequence
||
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<pre>MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG SGDIIDNIKV
<pre>MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG SGDIIDNIKV
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AMTLLTTLAV ADDIKLVDWL QQRLGLLEQR DTAMLHRLLH DIEKNITK</pre>
AMTLLTTLAV ADDIKLVDWL QQRLGLLEQR DTAMLHRLLH DIEKNITK</pre>
|-
|-
!align=left |Length
!align=left {{table heading style}} |Length
||
||
228
228
|-
|-
!align=left |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
||
||
25.259 kDa
25.259 kDa
|-
|-
!align=left |pI
!align=left {{table heading style}} |pI
||
||
5.9 (calculated)
5.9 (calculated)
|-
|-
!align=left |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
||
||
25,440 - 25,565 (calc based on 6 Y, 3 W, and 1 C residues)
25,440 - 25,565 (calc based on 6 Y, 3 W, and 1 C residues)
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!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-  
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Removed
Removed
|
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[http://www.uniprot.org/uniprot/P0A6T9#section_features UniProt:P0A6T9]  
[http://www.uniprot.org/uniprot/P0A6T9#section_features UniProt:P0A6T9]
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|-
|
Domain
|
8..129
|
[http://pfam.janelia.org/family/PF01597 PF01597  Glycine cleavage H-protein]
|
 
|
PMID:19920124


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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26176.U46aac5b93decc&page=26176&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table]</span> || || || ||
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<beststructure>
<beststructure>
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</beststructure>
</beststructure>
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!align=left |Models
!align=left {{table heading style}} |Models
||
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View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P0A6T9 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0A6T9 PSI-PRED (P0A6T9)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0A6T9 Genomes TO Protein (P0A6T9)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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!|Resource type!!Source!!Notes/Reference


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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U46aac5ba68152"  class=" tableEdit Gene_accessions_table"   
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16130806 GI:16130806]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_417380.1 GeneID:947393]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=GCVH-MONOMER EcoCyc:GCVH-MONOMER]
[[ASAP]]
|
|
ASAP:ABE-0009534
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
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[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P0A6T9 UniProtKB/Swiss-Prot:P0A6T9]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EcoCyc]]
|
EcoCyc:EG10371
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG10371
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947393 GeneID:947393]
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://pfam.sanger.ac.uk/family?acc=PF01597 PFAM:PF01597]
RegulonDB:ECK120000364
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
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|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
[[EchoBASE]]
|
|
[[uniprot:P0A6T9|UniProt:P0A6T9]]
EchoBASE:EB0366
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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[[Category:Proteins]]
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0009063_!_amino acid catabolic process]][[Category:GO:0009257_!_10-formyltetrahydrofolate biosynthetic process]]
[[Category:RefGenome_Annotated_Gene]]
[[Category:RefGenome_Annotated_Gene]]

Latest revision as of 20:57, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GcvH

Synonyms

glycine cleavage complex lipoylprotein [1], Gcv [2][1], B2904 [2][1] , ECK2899, JW2872, b2904

Product description

H-protein-(dihydrolipoyl)lysine[2][3], H-protein-S-(aminomethyldihydrolipoyl)lysine[2][3]


Glycine cleavage, carrier of aminomethyl group[4]

EC number (for enzymes)


edit table


Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019464

glycine decarboxylation via glycine cleavage system

PMID:1802033[5]

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P11183

P

complete

GO:0005739

mitochondrion

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002930

C

Seeded from EcoCyc (v14.0)

complete

GO:0031405

lipoic acid binding

PMID:1802033[5]

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P11183

F

complete

GO:0005960

glycine cleavage complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002930

C

Seeded from EcoCyc (v14.0)

complete

GO:0006546

glycine catabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002930

P

Seeded from EcoCyc (v14.0)

complete

GO:0019464

glycine decarboxylation via glycine cleavage system

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00272

P

Seeded from EcoCyc (v14.0)

complete

GO:0031405

lipoic acid binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0450

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:15642479[6]

IDA: Inferred from Direct Assay

C

complete

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Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

GcvT

Cross-linking of purified GcvT and GcvH in the presence of folate substrates. Cross-linking was done with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide.

PMID:10491090[7]


edit table


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
edit table

<protect>

Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MRITIILVAP ARAENIGAAA RAMKTMGFSD LRIVDSQAHL EPATRWVAHG SGDIIDNIKV
FPTLAESLHD VDFTVATTAR SRAKYHYYAT PVELVPLLEE KSSWMSHAAL VFGREDSGLT
NEELALADVL TGVPMVADYP SLNLGQAVMV YCYQLATLIQ QPAKSDATAD QHQLQALRER
AMTLLTTLAV ADDIKLVDWL QQRLGLLEQR DTAMLHRLLH DIEKNITK
Length

228

Mol. Wt

25.259 kDa

pI

5.9 (calculated)

Extinction coefficient

25,440 - 25,565 (calc based on 6 Y, 3 W, and 1 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6T9

Domain

8..129

PF01597 Glycine cleavage H-protein

PMID:19920124[8]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gcvH taxon=562,83333 </beststructure>

Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130806

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947393

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009534

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6T9

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10371

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10371

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947393

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000364

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0366

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Stauffer, LT et al. (1991) An Escherichia coli protein with homology to the H-protein of the glycine cleavage enzyme complex from pea and chicken liver. DNA Seq. 2 13-7 PubMed
  6. ↑ Nesbitt, NM et al. (2005) Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase. Protein Expr. Purif. 39 269-82 PubMed
  7. ↑ Okamura-Ikeda, K et al. (1999) The amino-terminal region of the Escherichia coli T-protein of the glycine cleavage system is essential for proper association with H-protein. Eur. J. Biochem. 264 446-53 PubMed
  8. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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