nrdE:Gene Product(s): Difference between revisions

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*  
*  
****************************************************************************************** -->{{Product_nomenclature_help}}
****************************************************************************************** -->{{Product_nomenclature_help}}
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|-
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!align=left {{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
||
||
ribonucleoside-diphosphate reductase 2, alpha subunit<ref name='LIB:Riley_2006'/>, B2675<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, NrdE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''ribonucleoside-diphosphate reductase 2, alpha subunit<ref name='LIB:Riley_2006'/>, B2675<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, NrdE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK2669, JW2650, b2675''
|-
|-
!align=left {{table heading style}} |Product description
!align=left {{table heading style}} |Product description
Line 57: Line 57:
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1]<ref name='LIB:Riley_2006'/>


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*  
*  
****************************************************************************************** -->{{Product_GO_help}}
****************************************************************************************** -->{{Product_GO_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X46aab5f36eaf2"  class=" tableEdit GO_table_product dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|


|
|
GO:0009263
GO:0005737
|
cytoplasm
|
PMID:7809142
|
ISA: Inferred from Sequence Alignment
|
UniProtKB:Q08698
|
C
|
 
|
complete
|-
|
 
|
GO:0015949
|
|
deoxyribonucleotide biosynthetic process
nucleobase, nucleoside and nucleotide interconversion
|
|
PMID:7809142
PMID:7809142
Line 95: Line 114:
P
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
A plasmid expressing nrdEFHI genes from Salmonella was capable of complementing nrdAB- mutations in E. coli.
|
|
complete  
complete  
Line 102: Line 121:


|
|
GO:0000166
GO:0005524
|
|
nucleotide binding
ATP binding
|
|
GO_REF:0000004
GO:0016959
|
|
IEA: Inferred from Electronic Annotation
ISA: Inferred from Sequence Alignment
|
|
SP_KW:KW-0547
UniProtKB:Q08698
|
|
F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
 
|
|
complete  
complete  
|-  
|-  
|
|
 
Contributes to
|
|
GO:0003824
GO:0004748
|
|
catalytic activity
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
|
|
GO_REF:0000004
PMID:7809142
|
|
IEA: Inferred from Electronic Annotation
IGI: Inferred from Genetic Interaction
|
|
SP_KW:KW-0021
EcoliWiki:nrdA|EcoliWiki
 
 
|
|
F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
A plasmid expressing nrdEFHI genes from Salmonella was capable of complementing nrdAB- mutations in E. coli.
 
new GO replaces obsolete GO:0016959
|
|
complete  
complete  
|-  
|-  
|
|
 
Contributes to
|
|
GO:0004748
GO:0004748
Line 144: Line 167:
ribonucleoside-diphosphate reductase activity
ribonucleoside-diphosphate reductase activity
|
|
GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000003
PMID:7809142
|
|
IEA: Inferred from Electronic Annotation
IGI: Inferred from Genetic Interaction
|
|
InterPro:IPR000788<br />InterPro:IPR008926<br />InterPro:IPR013554<br />EC:1.17.4.1
EcoliWiki:nrdA|EcoliWiki:nrdB
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete  
complete  
Line 159: Line 182:


|
|
GO:0005515
GO:0005971
|
|
protein binding
ribonucleoside-diphosphate reductase complex
|
|
GO_REF:0000002<br />GO_REF:0000002
PMID:7809142
|
|
IEA: Inferred from Electronic Annotation
IGI: Inferred from Genetic Interaction
|
|
InterPro:IPR008926<br />InterPro:IPR013554
EcoliWiki:nrdA|EcoliWiki:nrdB
|
|
F
C
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
 
|
|
complete  
complete  
Line 178: Line 201:


|
|
GO:0005524
GO:0000166
|
|
ATP binding
nucleotide binding
|
|
GO_REF:0000004
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0067
SP_KW:KW-0547
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 197: Line 220:


|
|
GO:0005971
GO:0004748
|
|
ribonucleoside-diphosphate reductase complex
ribonucleoside-diphosphate reductase activity
|
|
GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR000788<br />InterPro:IPR008926<br />InterPro:IPR013346<br />InterPro:IPR013554
InterPro:IPR000788
|
|
C
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 216: Line 239:


|
|
GO:0006260
GO:0009263
|
|
DNA replication
deoxyribonucleotide biosynthetic process
|
|
GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000004
PMID:7809142
|
|
IEA: Inferred from Electronic Annotation
IGI: Inferred from Genetic Interaction
|
|
InterPro:IPR000788<br />InterPro:IPR008926<br />InterPro:IPR013554<br />SP_KW:KW-0235
EcoliWiki:nrdA|EcoliWiki:nrdB
|
|
P
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete  
complete  
Line 235: Line 258:


|
|
GO:0016491
GO:0004748
|
|
oxidoreductase activity
ribonucleoside-diphosphate reductase activity
|
|
GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0560
InterPro:IPR008926
|
|
F
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 254: Line 277:


|
|
GO:0005737
GO:0004748
|
|
cytoplasm
ribonucleoside-diphosphate reductase activity
|
|
PMID:7809142
GOA:interpro<br />GO_REF:0000002
|
|
ISA: Inferred from Sequence Alignment
IEA: Inferred from Electronic Annotation
|
|
UniProtKB:Q08698
InterPro:IPR013509
|
|
C
F
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 273: Line 296:


|
|
GO:0055114
GO:0004748
|
|
oxidation reduction
ribonucleoside-diphosphate reductase activity
|
|
GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0560
InterPro:IPR013554
|
|
P
F
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 292: Line 315:


|
|
GO:0000166
GO:0004748
|
|
nucleotide binding
ribonucleoside-diphosphate reductase activity
|
|
GO_REF:0000004
GOA:spec<br />GO_REF:0000003
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0547
EC:1.17.4.1
|
|
F
F
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 311: Line 334:


|
|
GO:0003824
GO:0005515
|
|
catalytic activity
protein binding
|
|
GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0021
InterPro:IPR008926
|
|
F
F
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 330: Line 353:


|
|
GO:0004748
GO:0005515
|
|
ribonucleoside-diphosphate reductase activity
protein binding
|
|
GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000003
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR000788<br />InterPro:IPR008926<br />InterPro:IPR013509<br />InterPro:IPR013554<br />EC:1.17.4.1
InterPro:IPR013554
|
|
F
F
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 349: Line 372:


|
|
GO:0005515
GO:0005524
|
|
protein binding
ATP binding
|
|
GO_REF:0000002<br />GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR008926<br />InterPro:IPR013554
InterPro:IPR013509
|
|
F
F
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 372: Line 395:
ATP binding
ATP binding
|
|
GO_REF:0000002<br />GO_REF:0000004
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR013509<br />SP_KW:KW-0067
SP_KW:KW-0067
|
|
F
F
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005971
|
ribonucleoside-diphosphate reductase complex
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000788
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005971
|
ribonucleoside-diphosphate reductase complex
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR008926
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
 
|
GO:0005971
|
ribonucleoside-diphosphate reductase complex
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR013346
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 387: Line 467:


|
|
GO:0009263
GO:0005971
|
|
deoxyribonucleotide biosynthetic process
ribonucleoside-diphosphate reductase complex
|
|
PMID:7809142
GOA:interpro<br />GO_REF:0000002
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
EcoliWiki:nrdA|EcoliWiki:nrdB
InterPro:IPR013554
|
|
P
C
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 406: Line 486:


|
|
GO:0015949
GO:0006260
|
|
nucleobase, nucleoside and nucleotide interconversion
DNA replication
|
|
PMID:7809142
GOA:interpro<br />GO_REF:0000002
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
EcoliWiki:nrdA|EcoliWiki:nrdB
InterPro:IPR000788
|
|
P
P
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 429: Line 509:
DNA replication
DNA replication
|
|
GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000002<br />GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR000788<br />InterPro:IPR008926<br />InterPro:IPR013509<br />InterPro:IPR013554<br />SP_KW:KW-0235
InterPro:IPR008926
|
|
P
P
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 444: Line 524:


|
|
GO:0016491
GO:0006260
|
|
oxidoreductase activity
DNA replication
|
|
GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0560
InterPro:IPR013509
|
|
F
P
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 463: Line 543:


|
|
GO:0055114
GO:0006260
|
|
oxidation reduction
DNA replication
|
|
GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0560
InterPro:IPR013554
|
|
P
P
|
|
Seeded from EcoCyc
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 482: Line 562:


|
|
GO:0015949
GO:0006260
|
|
nucleobase, nucleoside and nucleotide interconversion
DNA replication
|
|
PMID:7809142
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
EcoliWiki:nrdA|EcoliWiki:nrdB
SP_KW:KW-0235
|
|
P
P
|
|
A plasmid expressing nrdEFHI genes from Salmonella was capable of complementing nrdAB- mutations in E. coli.
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 501: Line 581:


|
|
GO:0005524
GO:0016491
|
|
ATP binding
oxidoreductase activity
|
|
GO:0016959
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
ISA: Inferred from Sequence Alignment
IEA: Inferred from Electronic Annotation
|
|
UniProtKB:Q08698
SP_KW:KW-0560
|
|
F
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|
|


|
GO:0055114
|
oxidation reduction
|
GOA:interpro<br />GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR008926
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
Contributes to
 
|
|
GO:0016959
GO:0055114
|
|
class I ribonucleotide reductase activity
oxidation reduction
|
|
PMID:7809142
GOA:interpro<br />GO_REF:0000002
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
EcoliWiki:nrdA|EcoliWiki:nrdB
InterPro:IPR013346
|
|
F
P
|
|
A plasmid expressing nrdEFHI genes from Salmonella was capable of complementing nrdAB- mutations in E. coli.
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
Contributes to
 
|
|
GO:0004748
GO:0055114
|
|
ribonucleoside-diphosphate reductase activity
oxidation reduction
|
|
PMID:7809142
GOA:interpro<br />GO_REF:0000002
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
EcoliWiki:nrdA|EcoliWiki:nrdB
InterPro:IPR013509
|
|
F
P
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 558: Line 657:


|
|
GO:0005971
GO:0055114
|
|
ribonucleoside-diphosphate reductase complex
oxidation reduction
|
|
PMID:7809142
GOA:interpro<br />GO_REF:0000002
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
EcoliWiki:nrdA|EcoliWiki:nrdB
InterPro:IPR013554
|
|
C
P
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 577: Line 676:


|
|
GO:0009263
GO:0055114
|
|
deoxyribonucleotide biosynthetic process
oxidation reduction
|
|
PMID:7809142
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IGI: Inferred from Genetic Interaction
IEA: Inferred from Electronic Annotation
|
|
EcoliWiki:nrdA|EcoliWiki:nrdB
SP_KW:KW-0560
|
|
P
P
|
|
 
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  


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*  
*  
****************************************************************************************** -->{{Product_interactions_help}}
****************************************************************************************** -->{{Product_interactions_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="H46aab5ff885de"  class=" tableEdit Product_interactions_table dataTable"   
|- {{table heading style}}
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence
!|Partner Type!!Partner!!Notes!!References!!Evidence
Line 720: Line 819:
   
   


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*  
*  
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!|Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes


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|- {{table heading style}}
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!|Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-
|
Initiator Methionine
|
1
|
Removed (By similarity)
|
[http://www.uniprot.org/uniprot/P39452#section_features UniProt:P39452]
|
|-
|
Domain
|
98..172
|
[http://pfam.janelia.org/family/PF00317 PF00317  Ribonucleotide reductase, all-alpha domain]
|
 
|
PMID:19920124
|-
|
Domain
|
175..694
|
[http://pfam.janelia.org/family/PF02867 PF02867  Ribonucleotide reductase, barrel domain]
|
 
|
PMID:19920124
|-
|
Domain
|
16..97
|
[http://pfam.janelia.org/family/PF08343 PF08343  Ribonucleotide reductase N-terminal]
|
 
|
PMID:19920124


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View models at:
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://www.proteinmodelportal.org/query/uniprot/P39452 Protein Model Portal]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P39452 PSI-PRED (P39452)]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P39452 Genomes TO Protein (P39452)] (GTOP)
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16130589 GI:16130589]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_417161.1 GeneID:947155]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0008806
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=NRDE-MONOMER EcoCyc:NRDE-MONOMER]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P39452 UniProtKB/Swiss-Prot:P39452]
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://swissmodel.expasy.org/repository/ SwissModel] ([[SwissModel|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://swissmodel.expasy.org/repository/smr.php?job=2&sptr_ac=P39452 SwissModel:P39452]
EcoCyc:EG20257
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
[[EcoGene]]
|
|
[[uniprot:P39452|UniProt:P39452]]
EcoGene:EG20257
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[http://pfam.sanger.ac.uk/family?acc=PF00317 PFAM:PF00317]
[http://www.ncbi.nlm.nih.gov/gene?term=947155 GeneID:947155]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417161 RefSeq:NP_417161]
RegulonDB:ECK120002450
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://salilab.org/modbase/searchbyid?databaseID=P39452 ModBase:P39452]
EchoBASE:EB4158
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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{{BacktoTop}}
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[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0015949_!_nucleobase, nucleoside and nucleotide interconversion]][[Category:Complex:ribonucleoside-diphosphate reductase 2, &alpha; subunit dimer]][[Category:Complex:ribonucleoside-diphosphate reductase 2]]
[[Category:RefGenome_Annotated_Gene]]

Latest revision as of 20:54, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NrdE

Synonyms

ribonucleoside-diphosphate reductase 2, alpha subunit[1], B2675[2][1], NrdE[2][1] , ECK2669, JW2650, b2675

Product description

NrdE[2][3];

Component of ribonucleoside-diphosphate reductase 2, α subunit dimer[2][3]; ribonucleoside-diphosphate reductase 2[2][3]

EC number (for enzymes)
edit table


Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

PMID:7809142[4]

ISA: Inferred from Sequence Alignment

UniProtKB:Q08698

C

complete

GO:0015949

nucleobase, nucleoside and nucleotide interconversion

PMID:7809142[4]

IGI: Inferred from Genetic Interaction

EcoliWiki:nrdA|EcoliWiki:nrdB

P

A plasmid expressing nrdEFHI genes from Salmonella was capable of complementing nrdAB- mutations in E. coli.

complete

GO:0005524

ATP binding

GO:0016959

ISA: Inferred from Sequence Alignment

UniProtKB:Q08698

F

complete

Contributes to

GO:0004748

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

PMID:7809142[4]

IGI: Inferred from Genetic Interaction

EcoliWiki:nrdA|EcoliWiki


F

A plasmid expressing nrdEFHI genes from Salmonella was capable of complementing nrdAB- mutations in E. coli.

new GO replaces obsolete GO:0016959

complete

Contributes to

GO:0004748

ribonucleoside-diphosphate reductase activity

PMID:7809142[4]

IGI: Inferred from Genetic Interaction

EcoliWiki:nrdA|EcoliWiki:nrdB

F

complete

GO:0005971

ribonucleoside-diphosphate reductase complex

PMID:7809142[4]

IGI: Inferred from Genetic Interaction

EcoliWiki:nrdA|EcoliWiki:nrdB

C

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004748

ribonucleoside-diphosphate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000788

F

Seeded from EcoCyc (v14.0)

complete

GO:0009263

deoxyribonucleotide biosynthetic process

PMID:7809142[4]

IGI: Inferred from Genetic Interaction

EcoliWiki:nrdA|EcoliWiki:nrdB

P

complete

GO:0004748

ribonucleoside-diphosphate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008926

F

Seeded from EcoCyc (v14.0)

complete

GO:0004748

ribonucleoside-diphosphate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013509

F

Seeded from EcoCyc (v14.0)

complete

GO:0004748

ribonucleoside-diphosphate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013554

F

Seeded from EcoCyc (v14.0)

complete

GO:0004748

ribonucleoside-diphosphate reductase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.17.4.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008926

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013554

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013509

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005971

ribonucleoside-diphosphate reductase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000788

C

Seeded from EcoCyc (v14.0)

complete

GO:0005971

ribonucleoside-diphosphate reductase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008926

C

Seeded from EcoCyc (v14.0)

complete

GO:0005971

ribonucleoside-diphosphate reductase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013346

C

Seeded from EcoCyc (v14.0)

complete

GO:0005971

ribonucleoside-diphosphate reductase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013554

C

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000788

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008926

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013509

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013554

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0235

P

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008926

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013346

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013509

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013554

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of ribonucleoside-diphosphate reductase 2, α subunit dimer

could be indirect

Protein

intS

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

tusE

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

groL

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

yagA

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

ygeV

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

ydcS

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

ytfM

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

rplB

PMID:16606699[5]

Experiment(s):EBI-1143641

Protein

Subunits of ribonucleoside-diphosphate reductase 2

could be indirect


edit table


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MATTTAECLT QETMDYHALN AMLNLYDSAG RIQFDKDRQA VDAFIATHVR PNSVTFSSQQ
QRLNWLVNEG YYDESVLNRY SRDFVITLFT HAHTSGFRFQ TFLGAWKFYT SYTLKTFDGK
RYLEDFADRV TMVALTLAQG DETLALQLTD EMLSGRFQPA TPTFLNCGKQ QRGELVSCFL
LRIEDNMESI GRAVNSALQL SKRGGGVAFL LSNLREAGAP IKRIENQSSG VIPVMKMLED
AFSYANQLGA RQGAGAVYLH AHHPDILRFL DTKRENADEK IRIKTLSLGV VIPDITFHLA
KENAQMALFS PYDVERVYGK PFADVAISQH YDELVADERI RKKYLNARDF FQRLAEIQFE
SGYPYIMYED TVNRANPIAG RINMSNLCSE ILQVNSASEY DENLDYTRTG HDISCNLGSL
NIAHTMDSPD FARTVETAVR GLTAVSDMSH IRSVPSIEAG NAASHAIGLG QMNLHGYLAR
EGIAYGSPEA LDFTNLYFYA ITWHALRTSM LLARERGETF AGFKQSRYAS GEYFSQYLQG
NWQPKTAKVG ELFTRSGITL PTREMWAQLR DDVMRYGIYN QNLQAVPPTG SISYINHATS
SIHPIVAKVE IRKEGKTGRV YYPAPFMTNE NLALYQDAYE IGAEKIIDTY AEATRHVDQG
LSLTLFFPDT ATTRDINKAQ IYAWRKGIKT LYYIRLRQMA LEGTEIEGCV SCAL
Length

714

Mol. Wt

80.481 kDa

pI

6.5 (calculated)

Extinction coefficient

88,130 - 89,005 (calc based on 37 Y, 6 W, and 7 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References

Initiator Methionine

1

Removed (By similarity)

UniProt:P39452

Domain

98..172

PF00317 Ribonucleotide reductase, all-alpha domain

PMID:19920124[6]

Domain

175..694

PF02867 Ribonucleotide reductase, barrel domain

PMID:19920124[6]

Domain

16..97

PF08343 Ribonucleotide reductase N-terminal

PMID:19920124[6]

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<motif_map/>

Structure
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Structures

<beststructure> gene=nrdE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130589

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947155

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008806

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P39452

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG20257

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20257

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947155

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002450

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4158

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 Jordan, A et al. (1994) A second class I ribonucleotide reductase in Enterobacteriaceae: characterization of the Salmonella typhimurium enzyme. Proc. Natl. Acad. Sci. U.S.A. 91 12892-6 PubMed
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ 6.0 6.1 6.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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