maeB:Gene Product(s): Difference between revisions

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|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Gene Product Resources|Resources]]
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{|{{Prettytable}}
{|  id="H46aaa8f58949d"  class=" tableEdit Product_nomenclature_table" 
 
|-
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!{{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
|
||
MaeB product
MaeB  
|-
|-
!{{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
|
||
fused malic enzyme predicted oxidoreductase product<ref name='LIB:Riley_2006'/>, predicted phosphotransacetylase product<ref name='LIB:Riley_2006'/>, B2463 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YpfF product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MaeB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''fused malic enzyme predicted oxidoreductase<ref name='LIB:Riley_2006'/>, predicted phosphotransacetylase<ref name='LIB:Riley_2006'/>, B2463<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YpfF<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MaeB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK2458, JW2447, ypfF, b2463''
|-
|-
!{{table heading style}} |Product description
!align=left {{table heading style}} |Product description
|
||
MaeB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
MaeB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  


Component of ; [[:Category:Complex:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
Component of [[:Category:Complex:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


NADP-dependent malic enzyme; NADP-ME<ref name='LIB:EcoGene'/>
|-
|-
!{{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
|
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*
 
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<protect><!--Nomenclature_notes--></protect>
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===Notes===
===Notes===
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{{BacktoTop}}
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<protect>
<protect>
<big>'''Gene Ontology'''</big><br />{{Product_GO_help}}<!--box uid=421aa90e079fa326b6494f812ad13e79.25166.B46aaa8f709778-->
<big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.25166.B46aaa8f709778-->
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="B46aaa8f709778"  class=" tableEdit GO_table_product dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
|


|
|
[[Category:GO:0003824_!_catalytic_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0003824_!_catalytic_activity GO:0003824]
GO:0004473
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|
catalytic activity
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity
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|
GO_REF:0000004
GOA:spec<br />GO_REF:0000003
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|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0511
EC:1.1.1.40
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 99: Line 103:


|
|
[[Category:GO:0004473_!_malate_dehydrogenase_(oxaloacetate-decarboxylating)_(NADP+)_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0004473_!_malate_dehydrogenase_(oxaloacetate-decarboxylating)_(NADP+)_activity GO:0004473]
GO:0004473
|
|
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity
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GO_REF:0000003
PMID:11092847
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|
IEA: Inferred from Electronic Annotation
IMP: Inferred from Mutant Phenotype
|
|
EC:1.1.1.40
 
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 118: Line 122:


|
|
[[Category:GO:0005515_!_protein_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005515_!_protein_binding GO:0005515]
GO:0005829
|
|
protein binding
cytosol
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|
[[PMID:16606699]]<ref name=PMID:16606699/>
PMID:16858726
|
|
IPI: Inferred from Physical Interaction
IDA: Inferred from Direct Assay
|
|
UniProtKB:P18843
 
|
|
F
C
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 137: Line 141:


|
|
[[Category:GO:0008152_!_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008152_!_metabolic_process GO:0008152]
GO:0008152
|
|
metabolic process
metabolic process
|
|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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P
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
GO:0008152
|
|
acyltransferase activity
metabolic process
|
|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR002505
InterPro:IPR016040
|
|
F
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
[[Category:GO:0016491_!_oxidoreductase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016491_!_oxidoreductase_activity GO:0016491]
GO:0008415
|
|
oxidoreductase activity
acyltransferase activity
|
|
GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0560
InterPro:IPR002505
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 194: Line 198:


|
|
[[Category:GO:0016616_!_oxidoreductase_activity,_acting_on_the_CH-OH_group_of_donors,_NAD_or_NADP_as_acceptor]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016616_!_oxidoreductase_activity,_acting_on_the_CH-OH_group_of_donors,_NAD_or_NADP_as_acceptor GO:0016616]
GO:0046872
|
|
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
metal ion binding
|
|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 213: Line 217:


|
|
[[Category:GO:0016616_!_oxidoreductase_activity,_acting_on_the_CH-OH_group_of_donors,_NAD_or_NADP_as_acceptor]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016616_!_oxidoreductase_activity,_acting_on_the_CH-OH_group_of_donors,_NAD_or_NADP_as_acceptor GO:0016616]
GO:0046872
|
|
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
metal ion binding
|
|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR012301
InterPro:IPR015884
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 232: Line 236:


|
|
[[Category:GO:0016616_!_oxidoreductase_activity,_acting_on_the_CH-OH_group_of_donors,_NAD_or_NADP_as_acceptor]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016616_!_oxidoreductase_activity,_acting_on_the_CH-OH_group_of_donors,_NAD_or_NADP_as_acceptor GO:0016616]
GO:0046872
|
|
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
metal ion binding
|
|
GO_REF:0000002
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR012302
SP_KW:KW-0479
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
Line 251: Line 255:


|
|
[[Category:GO:0046872_!_metal_ion_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0046872_!_metal_ion_binding GO:0046872]
GO:0051287
|
|
metal ion binding
NAD or NADH binding
|
|
GO_REF:0000002
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR012188
InterPro:IPR012302
|
|
F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
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|
|
[[Category:GO:0046872_!_metal_ion_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0046872_!_metal_ion_binding GO:0046872]
GO:0055114
|
|
metal ion binding
oxidation reduction
|
|
GO_REF:0000004
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
SP_KW:KW-0479
InterPro:IPR012188
|
|
F
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  
|-  
|-  
|
|
 
|
|
[[Category:GO:0006094_!_gluconeogenesis]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006094_!_gluconeogenesis GO:0006094]
GO:0055114
|
|
gluconeogenesis
oxidation reduction
|
|
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR015884
|
|
P  
P
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.  
Seeded from [[EcoCyc]] (v14.0)
|
|
required fields missing 
complete
|-  
|-  
|
|


|
|
[[Category:GO:0051287_!_NAD_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0051287_!_NAD_binding GO:0051287]
GO:0055114
|
|
NAD binding
oxidation reduction
|
|
GO_REF:0000002
GOA:spkw<br />SP_KW:GO_REF:0000004
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
|
|
InterPro:IPR012302
SP_KW:KW-0560
|
|
F
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete  
complete  


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{|class = 'sortable' {{Prettytable}} id='169551'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z46aaa8fa1c7a9"  class=" tableEdit Product_interactions_table dataTable" 
|- {{table heading style}}
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!Partner Type!!Partner!!Notes
!|Partner Type!!Partner!!Notes!!References!!Evidence
|-
|-  
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Protein
Protein
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Subunits of [[:Category:Complex:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)]]  
Subunits of [[:Category:Complex:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)|malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)]]  
|
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could be indirect   
could be indirect  
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|
   
|-
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Protein
|
cca<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-881656<br />
|-
|
Protein
|
hrpA<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-881656<br />
|-
|
Protein
|
ynbB<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-889090<br />
|-
|
Protein
|
nrdA<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-889090<br />
|-
|
Protein
|
ycjZ<br />
|
 
|
PMID:15690043
|
Experiment(s):EBI-889090<br />
|-
|
Protein
|
nadE<br />
|
 
|
PMID:16606699
|
Experiment(s):EBI-1142921<br />
|-
|
Protein
|
nrdA
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.6
|-
|
Protein
|
ynbB
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.6
|-
|
Protein
|
ycjZ
|
 
|
PMID:19402753
|
LCMS(ID Probability):99.6
 
|- class="tableEdit_footer"
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<!--Function_notes--></protect>
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===Notes===
===Notes===
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!Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
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{|{{Prettytable}}
{|   id="F46aaa8fc741f2"  class=" tableEdit Product_phys_prop_table" 
 
|-
!align=left {{table heading style}} |Name
||
 
|-
|-
!{{table heading style}} |Sequence
!align=left {{table heading style}} |Sequence
|
||
[http://biocyc.org/ECOLI/sequence-aa?object=MALIC-NADP-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=MALIC-NADP-MONOMER at EcoCyc]  
<pre>MDDQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI EKDPLKAYKY
<pre>MDDQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI EKDPLKAYKY
Line 407: Line 521:
GPVLMGVAKP VHVLTPIASV RRIVNMVALA VVEAQTQPL</pre>  
GPVLMGVAKP VHVLTPIASV RRIVNMVALA VVEAQTQPL</pre>  
|-
|-
!{{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Length
|
||
82.42 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
759
|-
|-
!{{table heading style}} |pI
!align=left {{table heading style}} |Mol. Wt
|
||
82.417 kDa
|-
|-
!{{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |pI
|
||
30830 - 31955 (calc based on 17 Y, 1 W, and 9 C residues)  
5.3 (calculated)
|-
|-
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!align=left {{table heading style}} |Extinction coefficient
||
30,830 - 31,955 (calc based on 17 Y, 1 W, and 9 C residues)
 
|- class="tableEdit_footer"
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{{Product_physical_properties_help}}


<big>'''Domains/Motifs/Modification Sites'''</big>
 
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<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table>
<tr align="left" valign="top">
<td><!--box uid=421aa90e079fa326b6494f812ad13e79.25166.L46aaa8fce2c24-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="L46aaa8fce2c24"  class=" tableEdit Product_motif_table dataTable" 
|- {{table heading style}}
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
!|Type!!Residues!!Description!!Notes!!References
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|
Domain
|
18..151
|
[http://pfam.janelia.org/family/PF00390 PF00390  Malic enzyme, N-terminal domain]
|
 
|
PMID:19920124
|-
|
Domain
|
163..401
|
[http://pfam.janelia.org/family/PF03949 PF03949  Malic enzyme, NAD binding domain]
|
 
|
PMID:19920124
|-
|
Domain
|
429..751
|
[http://pfam.janelia.org/family/PF01515 PF01515  Phosphate acetyl/butaryl transferase]
|
 
|
PMID:19920124
 
|- class="tableEdit_footer"
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<td>
<!-- motifs column 2-->
<motif_map/>
</td>
</tr>
</table>


<span id="Structure"></span><big>'''Structure'''</big>
<table><tr valign='top'><td>
<span id='Structure'></span><big>'''Structure'''</big><br />
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|-
|-
!align=left {{table heading style}} |Structures
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=maeB
taxon=562,83333
</beststructure>
|-
|-
!align=left {{table heading style}} | Models
!align=left {{table heading style}} |Models
||
View models at:
* [http://www.proteinmodelportal.org/query/uniprot/P36930 Protein Model Portal]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P76558 PSI-PRED (P76558)]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P76558 Genomes TO Protein (P76558)] (GTOP)


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<!--Structure_notes--></protect>
</td><td></protect>
==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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===Notes===
===Notes===


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===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='169555'
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|- {{table_heading_style}}
|- {{table_heading_style}}
!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16130388 GI:16130388]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_416958.1 GeneID:946947]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0008111
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P76558 UniProtKB/Swiss-Prot:P76558]
|
Escherichia coli str. K-12 substr. MG1655
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=MALIC-NADP-MONOMER EcoCyc:MALIC-NADP-MONOMER] 
EcoCyc:G7293
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
[[EcoGene]]
|
|
[[uniprot:P76558|UniProt:P76558]] 
EcoGene:EG14193
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[http://pfam.sanger.ac.uk/family?acc=PF00390 PFAM:PF00390]
[http://www.ncbi.nlm.nih.gov/gene?term=946947 GeneID:946947]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_416958 RefSeq:NP_416958] 
RegulonDB:ECK120003867
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://salilab.org/modbase/searchbyid?databaseID=P76558 ModBase:P76558] 
EchoBASE:EB3945
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Notes===
===Notes===
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{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</noinclude>
===Categories===
===Categories===
{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0006094_!_gluconeogenesis]][[Category:Complex:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)]]

Latest revision as of 20:51, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MaeB

Synonyms

fused malic enzyme predicted oxidoreductase[1], predicted phosphotransacetylase[1], B2463[2][1], YpfF[2][1], MaeB[2][1] , ECK2458, JW2447, ypfF, b2463

Product description

MaeB[2][3];

Component of malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)[2][3]

NADP-dependent malic enzyme; NADP-ME[4]

EC number (for enzymes)


edit table


Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004473

malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.40

F

Seeded from EcoCyc (v14.0)

complete

GO:0004473

malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity

PMID:11092847[5]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[6]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002505

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

P

Seeded from EcoCyc (v14.0)

complete

GO:0008415

acyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002505

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012188

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015884

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012302

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012188

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015884

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

could be indirect

Protein

cca

PMID:15690043[7]

Experiment(s):EBI-881656

Protein

hrpA

PMID:15690043[7]

Experiment(s):EBI-881656

Protein

ynbB

PMID:15690043[7]

Experiment(s):EBI-889090

Protein

nrdA

PMID:15690043[7]

Experiment(s):EBI-889090

Protein

ycjZ

PMID:15690043[7]

Experiment(s):EBI-889090

Protein

nadE

PMID:16606699[8]

Experiment(s):EBI-1142921

Protein

nrdA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

ynbB

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

ycjZ

PMID:19402753[9]

LCMS(ID Probability):99.6

edit table


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
edit table


Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MDDQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI EKDPLKAYKY
TARGNLVAVI SNGTAVLGLG NIGALAGKPV MEGKGVLFKK FAGIDVFDIE VDELDPDKFI
EVVAALEPTF GGINLEDIKA PECFYIEQKL RERMNIPVFH DDQHGTAIIS TAAILNGLRV
VEKNISDVRM VVSGAGAAAI ACMNLLVALG LQKHNIVVCD SKGVIYQGRE PNMAETKAAY
AVVDDGKRTL DDVIEGADIF LGCSGPKVLT QEMVKKMARA PMILALANPE PEILPPLAKE
VRPDAIICTG RSDYPNQVNN VLCFPFIFRG ALDVGATAIN EEMKLAAVRA IAELAHAEQS
EVVASAYGDQ DLSFGPEYII PKPFDPRLIV KIAPAVAKAA MESGVATRPI ADFDVYIDKL
TEFVYKTNLF MKPIFSQARK APKRVVLPEG EEARVLHATQ ELVTLGLAKP ILIGRPNVIE
MRIQKLGLQI KAGVDFEIVN NESDPRFKEY WTEYFQIMKR RGVTQEQAQR ALISNPTVIG
AIMVQRGEAD AMICGTVGDY HEHFSVVKNV FGYRDGVHTA GAMNALLLPS GNTFIADTYV
NDEPDAEELA EITLMAAETV RRFGIEPRVA LLSHSNFGSS DCPSSSKMRQ ALELVRERAP
ELMIDGEMHG DAALVEAIRN DRMPDSSLKG SANILVMPNM EAARISYNLL RVSSSEGVTV
GPVLMGVAKP VHVLTPIASV RRIVNMVALA VVEAQTQPL
Length

759

Mol. Wt

82.417 kDa

pI

5.3 (calculated)

Extinction coefficient

30,830 - 31,955 (calc based on 17 Y, 1 W, and 9 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

18..151

PF00390 Malic enzyme, N-terminal domain

PMID:19920124[10]

Domain

163..401

PF03949 Malic enzyme, NAD binding domain

PMID:19920124[10]

Domain

429..751

PF01515 Phosphate acetyl/butaryl transferase

PMID:19920124[10]

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=maeB taxon=562,83333 </beststructure>

Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130388

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946947

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008111

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P76558

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7293

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14193

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946947

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003867

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3945

Escherichia coli str. K-12 substr. MG1655

edit table


Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ van der Rest, ME et al. (2000) Functions of the membrane-associated and cytoplasmic malate dehydrogenases in the citric acid cycle of Escherichia coli. J. Bacteriol. 182 6892-9 PubMed
  6. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ 9.0 9.1 9.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ 10.0 10.1 10.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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