mfd:Gene Product(s): Difference between revisions
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!align=left {{table heading style}} |Synonyms | !align=left {{table heading style}} |Synonyms | ||
|| | || | ||
transcription-repair coupling factor<ref name='LIB:Riley_2006'/>, B1114<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Mfd<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, TRCF<ref name='LIB:EcoCyc11.1'/><ref name='LIB:Riley_2006'/> | ''transcription-repair coupling factor<ref name='LIB:Riley_2006'/>, B1114<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Mfd<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, TRCF<ref name='LIB:EcoCyc11.1'/><ref name='LIB:Riley_2006'/> , ECK1100, JW1100, b1114'' | ||
|- | |- | ||
!align=left {{table heading style}} |Product description | !align=left {{table heading style}} |Product description | ||
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transcription-repair coupling factor<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/> | transcription-repair coupling factor<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/> | ||
Transcription repair coupling factor<ref name='LIB:EcoGene'/> | |||
|- | |- | ||
!align=left {{table heading style}} |EC number (for enzymes) | !align=left {{table heading style}} |EC number (for enzymes) | ||
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<protect><!--Nomenclature_notes--></protect> | <protect><!--Nomenclature_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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{{BacktoTop}} | {{BacktoTop}} | ||
<protect> | <protect> | ||
<big>'''Gene Ontology'''</big><br /> | <big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.22136.P46aa57867c376--> | ||
<!-- | <!-- | ||
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****************************************************************************************** -->{{Product_GO_help}} | ****************************************************************************************** -->{{Product_GO_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46aa57867c376" class=" tableEdit GO_table_product dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Qualifier!!GO ID!!GO term name!!Reference | !|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
|- | |||
| | |||
| | |||
GO:0006281 | |||
| | |||
DNA repair | |||
| | |||
PMID:1763073 | |||
| | |||
IMP: Inferred from Mutant Phenotype | |||
| | |||
| | |||
P | |||
| | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0006355 | |||
| | |||
regulation of transcription, DNA-dependent | |||
| | |||
PMID:8465200 | |||
| | |||
IDA: Inferred from Direct Assay | |||
| | |||
| | |||
P | |||
| | |||
| | |||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0006974 | |||
| | |||
response to DNA damage stimulus | |||
| | | | ||
PMID:8465200 | |||
| | | | ||
IDA: Inferred from Direct Assay | |||
| | | | ||
| | |||
P | |||
| | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0000716 | |||
| | |||
transcription-coupled nucleotide-excision repair, DNA damage recognition | |||
| | |||
PMID:8465200 | |||
| | |||
IDA: Inferred from Direct Assay | |||
| | |||
| | |||
P | |||
| | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0000166 | |||
| | |||
nucleotide binding | |||
| | |||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
SP_KW:KW-0547 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 99: | Line 177: | ||
| | | | ||
GO:0003676 | |||
| | | | ||
nucleic acid binding | |||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR001650 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 118: | Line 196: | ||
| | | | ||
GO:0003676 | |||
| | | | ||
nucleic acid binding | nucleic acid binding | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR011545 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 137: | Line 215: | ||
| | | | ||
GO:0003677 | |||
| | | | ||
DNA binding | DNA binding | ||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0238 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 156: | Line 234: | ||
| | | | ||
GO:0003684 | |||
| | | | ||
damaged DNA binding | damaged DNA binding | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR004576 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 175: | Line 253: | ||
| | | | ||
GO:0003684 | |||
| | |||
damaged DNA binding | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR005118 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0003700 | |||
| | | | ||
transcription factor activity | transcription factor activity | ||
| | | | ||
[ | GOA:interpro<br />GO_REF:0000002 | ||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR003711 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0004386 | |||
| | |||
helicase activity | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR001650 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0004386 | |||
| | |||
helicase activity | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR004576 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0004386 | |||
| | |||
helicase activity | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR005118 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 194: | Line 348: | ||
| | | | ||
GO:0004386 | |||
| | | | ||
helicase activity | helicase activity | ||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0347 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 213: | Line 367: | ||
| | | | ||
GO:0005524 | |||
| | | | ||
ATP binding | ATP binding | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR001650 | |||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 232: | Line 386: | ||
| | | | ||
[[ | GO:0005524 | ||
| | |||
ATP binding | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR004576 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0005524 | |||
| | |||
ATP binding | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR005118 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0005524 | |||
| | |||
ATP binding | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR011545 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0005524 | |||
| | |||
ATP binding | |||
| | |||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
SP_KW:KW-0067 | |||
| | |||
F | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0006281 | |||
| | | | ||
DNA repair | DNA repair | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR004576 | |||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 251: | Line 481: | ||
| | | | ||
GO:0006281 | |||
| | | | ||
DNA repair | |||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR005118 | |||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 270: | Line 500: | ||
| | | | ||
GO:0006281 | |||
| | | | ||
DNA repair | |||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0234 | |||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 289: | Line 519: | ||
| | | | ||
GO:0006355 | |||
| | | | ||
regulation of transcription, DNA-dependent | |||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR003711 | |||
| | | | ||
P | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
| Line 308: | Line 538: | ||
| | | | ||
GO:0006974 | |||
| | | | ||
response to DNA damage stimulus | |||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0227 | |||
| | | | ||
P | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
|- | |- | ||
| | | | ||
| | | | ||
GO:0008026 | |||
| | | | ||
ATP-dependent helicase activity | |||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
InterPro:IPR011545 | |||
| | | | ||
F | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0016787 | |||
| | | | ||
hydrolase activity | |||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
SP_KW:KW-0378 | |||
| | | | ||
F | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Y46aa578d15fd5" class=" tableEdit Product_interactions_table dataTable" | ||
|- {{table heading style}} | |- {{table heading style}} | ||
!|Partner Type!!Partner!!Notes!!References!!Evidence | !|Partner Type!!Partner!!Notes!!References!!Evidence | ||
| Line 382: | Line 612: | ||
Protein | Protein | ||
| | | | ||
tdcC<br /> | |||
| | |||
| | |||
PMID:15690043 | |||
| | |||
Experiment(s):EBI-893742<br /> | |||
|- | |||
| | |||
Protein | |||
| | |||
yffB<br /> | |||
| | |||
| | |||
PMID:15690043 | |||
| | |||
Experiment(s):EBI-893742<br /> | |||
|- | |||
| | |||
Protein | |||
| | |||
ihfA<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1138903<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rpsM<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1138903<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
yeeT<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1138903<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rplB<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1138903<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
stpA<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1138903<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
nfrA<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1138903<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
tdcC | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.0 | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
yffB | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.0 | |||
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<!--Function_notes--></protect> | <!--Function_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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* | * | ||
****************************************************************************************** -->{{Product_localization_help}} | ****************************************************************************************** -->{{Product_localization_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a7ea7d8df88" class=" tableEdit Product_localization_table" | ||
|- {{table heading style}} | |- {{table heading style}} | ||
!|Compartment!!Description!!Evidence!!Notes | !|Compartment!!Description!!Evidence!!Reference/Source!!Notes | ||
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<protect><!--Localization_notes--></protect> | <protect><!--Localization_notes--></protect> | ||
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****************************************************************************************** -->{{Product_physical_properties_help}} | ****************************************************************************************** -->{{Product_physical_properties_help}} | ||
{| | {| id="Y46aa578ed0dad" class=" tableEdit Product_phys_prop_table" | ||
|- | |||
!align=left {{table heading style}} |Name | |||
|| | |||
|- | |- | ||
| Line 527: | Line 791: | ||
NEKGGVIEFA EKNHVNPAWL IGLLQKQPQH YRLDGPTRLK FIQDLSERKT RIEWVRQFMR | NEKGGVIEFA EKNHVNPAWL IGLLQKQPQH YRLDGPTRLK FIQDLSERKT RIEWVRQFMR | ||
ELEENAIA</pre> | ELEENAIA</pre> | ||
|- | |||
!align=left {{table heading style}} |Length | |||
|| | |||
1,148 | |||
|- | |- | ||
!align=left {{table heading style}} |Mol. Wt | !align=left {{table heading style}} |Mol. Wt | ||
|| | || | ||
129. | 129.985 kDa | ||
|- | |- | ||
!align=left {{table heading style}} |pI | !align=left {{table heading style}} |pI | ||
|| | || | ||
6.1 (calculated) | |||
|- | |- | ||
!align=left {{table heading style}} |Extinction coefficient | !align=left {{table heading style}} |Extinction coefficient | ||
|| | || | ||
95,230 - 96,105 (calc based on 27 Y, 10 W, and 7 C residues) | |||
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<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span> | |||
<!--box uid=421aa90e079fa326b6494f812ad13e79.22136.Y46aa578f398c3--> | <span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table> | ||
<tr align="left" valign="top"> | |||
<td><!--box uid=421aa90e079fa326b6494f812ad13e79.22136.Y46aa578f398c3--> | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Y46aa578f398c3" class=" tableEdit Product_motif_table dataTable" | ||
|- {{table heading style}} | |- {{table heading style}} | ||
!|Type!!Residues!!Description!!Notes | !|Type!!Residues!!Description!!Notes!!References | ||
|- | |- | ||
| | | | ||
Domain | Domain | ||
| | | | ||
602..766 | |||
| | | | ||
[ | [http://pfam.janelia.org/family/PF00270 PF00270 DEAD/DEAH box helicase] | ||
| | | | ||
| | |||
PMID:19920124 | |||
|- | |- | ||
| | | | ||
Domain | Domain | ||
| | | | ||
832..907 | |||
| | | | ||
[ | [http://pfam.janelia.org/family/PF00271 PF00271 Helicase conserved C-terminal domain] | ||
| | | | ||
|-class= | | | ||
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.22136.Y46aa578f398c3&page=22136&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || | PMID:19920124 | ||
|- | |||
| | |||
Domain | |||
| | |||
476..573 | |||
| | |||
[http://pfam.janelia.org/family/PF02559 PF02559 CarD-like/TRCF domain] | |||
| | |||
| | |||
PMID:19920124 | |||
|- | |||
| | |||
Domain | |||
| | |||
1005..1105 | |||
| | |||
[http://pfam.janelia.org/family/PF03461 PF03461 TRCF domain] | |||
| | |||
| | |||
PMID:19920124 | |||
|- class="tableEdit_footer" | |||
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<td> | |||
<!-- motifs column 2--> | |||
<motif_map/> | |||
</td> | |||
</tr> | |||
</table> | |||
<span id= | <table><tr valign='top'><td> | ||
<span id='Structure'></span><big>'''Structure'''</big><br /> | |||
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!align=left {{table heading style}} |Structures | !align=left {{table heading style}} |Structures | ||
|| | || | ||
<beststructure>mfd | <beststructure> | ||
562</beststructure> | gene=mfd | ||
taxon=562,83333 | |||
</beststructure> | |||
|- | |- | ||
!align=left {{table heading style}} |Models | !align=left {{table heading style}} |Models | ||
|| | || | ||
View models at: | View models at: | ||
* [http:// | * [http://www.proteinmodelportal.org/query/uniprot/P30958 Protein Model Portal] | ||
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P30958 PSI-PRED (P30958)] | |||
* [http://bioinf.cs.ucl.ac.uk/ | * [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P30958 Genomes TO Protein (P30958)] (GTOP) | ||
* [http://spock.genes.nig.ac.jp/~genome/ | |||
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<!--Structure_notes--></protect> | </td><td></protect> | ||
==== Structure figures ==== | |||
<protect><!-- ***** PLEASE DO NOT EDIT BELOW THIS LINE ***** --> | |||
</td><tr><table><!--Structure_notes--></protect> | |||
===Notes=== | ===Notes=== | ||
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===Notes=== | ===Notes=== | ||
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|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | ||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/16129077 GI:16129077] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/protein/NP_415632.1 GeneID:945681] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[[ASAP]] | |||
| | |||
ASAP:ABE-0003763 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.uniprot.org/uniprot/P30958 UniProtKB/Swiss-Prot:P30958] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EcoCyc]] | |||
| | | | ||
EcoCyc:EG11619 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EcoGene]] | |||
| | | | ||
EcoGene:EG11619 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http://www.ncbi.nlm.nih.gov/ | [http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]]) | ||
| | | | ||
[http://www.ncbi.nlm.nih.gov/ | [http://www.ncbi.nlm.nih.gov/gene?term=945681 GeneID:945681] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120001567 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB1576 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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{{RefHelp}} | {{RefHelp}} | ||
<references/> | <references /> | ||
__NOTOC__ | __NOTOC__ | ||
===Categories=== | ===Categories=== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
[[Category:Proteins]] | [[Category:Proteins]] | ||
Latest revision as of 20:34, 17 February 2015
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Mfd |
|---|---|
| Synonyms |
transcription-repair coupling factor[1], B1114[2][1], Mfd[2][1], TRCF[3][1] , ECK1100, JW1100, b1114 |
| Product description |
transcription-repair coupling factor[2][3] Transcription repair coupling factor[4] |
| EC number (for enzymes) |
|
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006281 |
DNA repair |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0006355 |
regulation of transcription, DNA-dependent |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0006974 |
response to DNA damage stimulus |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0000716 |
transcription-coupled nucleotide-excision repair, DNA damage recognition |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003684 |
damaged DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003684 |
damaged DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003700 |
transcription factor activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004386 |
helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004386 |
helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004386 |
helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004386 |
helicase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006281 |
DNA repair |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006974 |
response to DNA damage stimulus |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008026 |
ATP-dependent helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016787 |
hydrolase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
tdcC |
Experiment(s):EBI-893742 | ||
|
Protein |
yffB |
Experiment(s):EBI-893742 | ||
|
Protein |
ihfA |
Experiment(s):EBI-1138903 | ||
|
Protein |
rpsM |
Experiment(s):EBI-1138903 | ||
|
Protein |
yeeT |
Experiment(s):EBI-1138903 | ||
|
Protein |
rplB |
Experiment(s):EBI-1138903 | ||
|
Protein |
stpA |
Experiment(s):EBI-1138903 | ||
|
Protein |
nfrA |
Experiment(s):EBI-1138903 | ||
|
Protein |
tdcC |
LCMS(ID Probability):99.0 | ||
|
Protein |
yffB |
LCMS(ID Probability):99.0 | ||
| edit table |
Notes
Mfd is involved in transcription-coupled repair[6][10]. Mfd is is an ATPase that can release stalled or paused RNA polymerases from DNA lesions, including thymine dimers and abasic sites[11]
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MPEQYRYTLP VKAGEQRLLG ELTGAACATL VAEIAERHAG PVVLIAPDMQ NALRLHDEIS QFTDQMVMNL ADWETLPYDS FSPHQDIISS RLSTLYQLPT MQRGVLIVPV NTLMQRVCPH SFLHGHALVM KKGQRLSRDA LRTQLDSAGY RHVDQVMEHG EYATRGALLD LFPMGSELPY RLDFFDDEID SLRVFDVDSQ RTLEEVEAIN LLPAHEFPTD KAAIELFRSQ WRDTFEVKRD PEHIYQQVSK GTLPAGIEYW QPLFFSEPLP PLFSYFPANT LLVNTGDLET SAERFQADTL ARFENRGVDP MRPLLPPQSL WLRVDELFSE LKNWPRVQLK TEHLPTKAAN ANLGFQKLPD LAVQAQQKAP LDALRKFLET FDGPVVFSVE SEGRREALGE LLARIKIAPQ RIMRLDEASD RGRYLMIGAA EHGFVDTVRN LALICESDLL GERVARRRQD SRRTINPDTL IRNLAELHIG QPVVHLEHGV GRYAGMTTLE AGGITGEYLM LTYANDAKLY VPVSSLHLIS RYAGGAEENA PLHKLGGDAW SRARQKAAEK VRDVAAELLD IYAQRAAKEG FAFKHDREQY QLFCDSFPFE TTPDQAQAIN AVLSDMCQPL AMDRLVCGDV GFGKTEVAMR AAFLAVDNHK QVAVLVPTTL LAQQHYDNFR DRFANWPVRI EMISRFRSAK EQTQILAEVA EGKIDILIGT HKLLQSDVKF KDLGLLIVDE EHRFGVRHKE RIKAMRANVD ILTLTATPIP RTLNMAMSGM RDLSIIATPP ARRLAVKTFV REYDSMVVRE AILREILRGG QVYYLYNDVE NIQKAAERLA ELVPEARIAI GHGQMREREL ERVMNDFHHQ RFNVLVCTTI IETGIDIPTA NTIIIERADH FGLAQLHQLR GRVGRSHHQA YAWLLTPHPK AMTTDAQKRL EAIASLEDLG AGFALATHDL EIRGAGELLG EEQSGSMETI GFSLYMELLE NAVDALKAGR EPSLEDLTSQ QTEVELRMPS LLPDDFIPDV NTRLSFYKRI ASAKTENELE EIKVELIDRF GLLPDPARTL LDIARLRQQA QKLGIRKLEG NEKGGVIEFA EKNHVNPAWL IGLLQKQPQH YRLDGPTRLK FIQDLSERKT RIEWVRQFMR ELEENAIA |
| Length |
1,148 |
| Mol. Wt |
129.985 kDa |
| pI |
6.1 (calculated) |
| Extinction coefficient |
95,230 - 96,105 (calc based on 27 Y, 10 W, and 7 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Selby, CP et al. (1991) Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor. Proc. Natl. Acad. Sci. U.S.A. 88 11574-8 PubMed
- ↑ 6.0 6.1 6.2 6.3 Selby, CP & Sancar, A (1993) Molecular mechanism of transcription-repair coupling. Science 260 53-8 PubMed
- ↑ 7.0 7.1 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 8.0 8.1 8.2 8.3 8.4 8.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 9.0 9.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Roberts, J & Park, JS (2004) Mfd, the bacterial transcription repair coupling factor: translocation, repair and termination. Curr. Opin. Microbiol. 7 120-5 PubMed
- ↑ Smith, AJ & Savery, NJ (2008) Effects of the bacterial transcription-repair coupling factor during transcription of DNA containing non-bulky lesions. DNA Repair (Amst.) 7 1670-9 PubMed
- ↑ 12.0 12.1 12.2 12.3 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0006281 ! DNA repair
- GO:0006355 ! regulation of transcription, DNA-templated
- GO:0006974 ! cellular response to DNA damage stimulus
- GO:0000716 ! transcription-coupled nucleotide-excision repair, DNA damage recognition
- GO:0000166 ! nucleotide binding
- GO:0003676 ! nucleic acid binding
- GO:0003677 ! DNA binding
- GO:0003684 ! damaged DNA binding
- GO:0003700 ! DNA-binding transcription factor activity
- GO:0004386 ! helicase activity
- GO:0005524 ! ATP binding
- GO:0008026 ! ATP-dependent helicase activity
- GO:0016787 ! hydrolase activity
- Proteins