mdfA:Gene Product(s): Difference between revisions
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{{Tab|Quickview| | {{Tab|Quickview|mdfA}}{{Tab|Gene|mdfA}}{{Tab On|Gene Product(s)|mdfA}}{{Tab|Expression|mdfA}}{{Tab|Evolution|mdfA}}{{Tab|On One Page|mdfA}}{{TabsBottom}} | ||
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{| | {| id="L46aa46bb4cfbb" class=" tableEdit Product_nomenclature_table" | ||
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!align=left {{table heading style}} |Standard name | !align=left {{table heading style}} |Standard name | ||
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MdfA | |||
|- | |- | ||
!align=left {{table heading style}} |Synonyms | !align=left {{table heading style}} |Synonyms | ||
|| | || | ||
multidrug efflux system protein<ref name='LIB:Riley_2006'/>, B0842<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CmlA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, | ''multidrug efflux system protein<ref name='LIB:Riley_2006'/>, B0842<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, CmlA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MdfA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cmr<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , cmlA, cmr, ECK0832, JW0826, b0842'' | ||
|- | |- | ||
!align=left {{table heading style}} |Product description | !align=left {{table heading style}} |Product description | ||
|| | || | ||
MdfA/ | MdfA/MdfA MFS multidrug transporter<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/> | ||
Transmembrane multidrug/chloramphenicol efflux transporter; confers alkaline pH tolerence<ref name='LIB:EcoGene'/> | Transmembrane multidrug/chloramphenicol efflux transporter; confers alkaline pH tolerence<ref name='LIB:EcoGene'/> | ||
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{| | {| id="C46aa46bc4c8b6" class=" tableEdit GO_table_product dataTable" | ||
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!|Qualifier!!GO ID!!GO term name!!Reference | !|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
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transporter activity | transporter activity | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR005829 | InterPro:IPR005829 | ||
| | | | ||
F | F | ||
| | | | ||
Seeded from EcoCyc < | Seeded from [[EcoCyc]] (v14.0) | ||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0005886 | |||
| | |||
plasma membrane | |||
| | |||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
SP_KW:KW-0997 | |||
| | |||
C | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0005886 | |||
| | |||
plasma membrane | |||
| | |||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
SP_KW:KW-1003 | |||
| | |||
C | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0006810 | |||
| | |||
transport | |||
| | |||
GOA:interpro<br />GO_REF:0000002 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
InterPro:IPR005829 | |||
| | |||
P | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | |||
complete | |||
|- | |||
| | |||
| | |||
GO:0006810 | |||
| | |||
transport | |||
| | |||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | |||
IEA: Inferred from Electronic Annotation | |||
| | |||
SP_KW:KW-0813 | |||
| | |||
P | |||
| | |||
Seeded from [[EcoCyc]] (v14.0) | |||
| | | | ||
complete | complete | ||
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membrane | membrane | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR005829 | InterPro:IPR005829 | ||
| | | | ||
C | C | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
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GO: | GO:0016020 | ||
| | | | ||
membrane | |||
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GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0472 | |||
| | | | ||
C | C | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
|- | |- | ||
| | | | ||
| | | | ||
GO: | GO:0016021 | ||
| | | | ||
integral to membrane | |||
| | | | ||
GOA:spkw<br />SP_KW:GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
SP_KW:KW-0812 | |||
| | | | ||
C | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO: | GO:0016021 | ||
| | | | ||
integral to membrane | |||
| | | | ||
GO_REF:0000023 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_SL:SL-9909 | |||
| | | | ||
C | C | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
|- | |- | ||
| | | | ||
| | | | ||
GO: | GO:0055085 | ||
| | | | ||
transmembrane transport | |||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | |||
| | | | ||
InterPro:IPR005829 | |||
| | | | ||
P | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO: | GO:0055085 | ||
| | | | ||
transport | transmembrane transport | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro: | InterPro:IPR011701 | ||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | complete | ||
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!|Partner Type!!Partner!!Notes!!References!!Evidence | !|Partner Type!!Partner!!Notes!!References!!Evidence | ||
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!|Compartment!!Description!!Evidence!!Notes | !|Compartment!!Description!!Evidence!!Reference/Source!!Notes | ||
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Daley et al. (2005) <ref name=PMID:15919996/> | Daley et al. (2005) <ref name=PMID:15919996/> | ||
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{| | {| id="X46aa46c4cb2f9" class=" tableEdit Product_phys_prop_table" | ||
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!align=left {{table heading style}} |Name | |||
|| | |||
|- | |- | ||
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VAAAATVISS HAYLWMTAGL SIYAFGIGLA NAGLVRLTLF ASDMSKGTVS AAMGMLQMLI | VAAAATVISS HAYLWMTAGL SIYAFGIGLA NAGLVRLTLF ASDMSKGTVS AAMGMLQMLI | ||
FTVGIEISKH AWLNGGNGLF NLFNLVNGIL WLSLMVIFLK DKQMGNSHEG</pre> | FTVGIEISKH AWLNGGNGLF NLFNLVNGIL WLSLMVIFLK DKQMGNSHEG</pre> | ||
|- | |||
!align=left {{table heading style}} |Length | |||
|| | |||
410 | |||
|- | |- | ||
!align=left {{table heading style}} |Mol. Wt | !align=left {{table heading style}} |Mol. Wt | ||
|| | || | ||
44. | 44.323 kDa | ||
|- | |- | ||
!align=left {{table heading style}} |pI | !align=left {{table heading style}} |pI | ||
|| | || | ||
9.1 (calculated) | |||
|- | |- | ||
!align=left {{table heading style}} |Extinction coefficient | !align=left {{table heading style}} |Extinction coefficient | ||
|| | || | ||
69,900 - 70,400 (calc based on 10 Y, 10 W, and 4 C residues) | |||
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{| | {| id="E46aa46c5322f6" class=" tableEdit Product_motif_table dataTable" | ||
|- {{table heading style}} | |- {{table heading style}} | ||
!|Type!!Residues!!Description!!Notes | !|Type!!Residues!!Description!!Notes!!References | ||
|- | |- | ||
| | | | ||
Domain | Domain | ||
| | | | ||
22..365 | |||
| | | | ||
[http://pfam.janelia.org/family/PF07690 PF07690 Major Facilitator Superfamily] | |||
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PMID:19920124 | |||
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</td> | </td> | ||
<td> | <td> | ||
<!-- motifs column 2--> | |||
<motif_map/> | |||
{{TMHMM Picture|cmr}} | {{TMHMM Picture|cmr}} | ||
</td> | </td> | ||
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</table> | </table> | ||
<span id= | <table><tr valign='top'><td> | ||
<span id='Structure'></span><big>'''Structure'''</big><br /> | |||
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!align=left {{table heading style}} |Structures | !align=left {{table heading style}} |Structures | ||
|| | || | ||
<beststructure>cmr | <beststructure> | ||
562</beststructure> | gene=cmr | ||
taxon=562,83333 | |||
</beststructure> | |||
|- | |- | ||
!align=left {{table heading style}} |Models | !align=left {{table heading style}} |Models | ||
|| | || | ||
View models at: | View models at: | ||
* [http:// | * [http://www.proteinmodelportal.org/query/uniprot/P0AEY8 Protein Model Portal] | ||
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0AEY8 PSI-PRED (P0AEY8)] | |||
* [http://bioinf.cs.ucl.ac.uk/ | * [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0AEY8 Genomes TO Protein (P0AEY8)] (GTOP) | ||
* [http://spock.genes.nig.ac.jp/~genome/ | |||
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<!--Structure_notes--></protect> | </td><td></protect> | ||
==== Structure figures ==== | |||
<protect><!-- ***** PLEASE DO NOT EDIT BELOW THIS LINE ***** --> | |||
</td><tr><table><!--Structure_notes--></protect> | |||
===Notes=== | ===Notes=== | ||
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{| | {| id="F46aa46c5ca60b" class=" tableEdit Product_resource_table" | ||
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!|Resource type!!Source!!Notes/Reference | !|Resource type!!Source!!Notes/Reference | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="O46aa46c6227db" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | ||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/16128810 GI:16128810] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/protein/NP_415363.1 GeneID:945448] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | [http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | ||
| | | | ||
[ | [http://www.uniprot.org/uniprot/P0AEY8 UniProtKB/Swiss-Prot:P0AEY8] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=945448 GeneID:945448] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB: | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE: | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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==References== | ==References== | ||
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===Categories=== | ===Categories=== | ||
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[[Category:Proteins]] | [[Category:Proteins]] | ||
Latest revision as of 16:28, 5 October 2013
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
MdfA |
|---|---|
| Synonyms |
multidrug efflux system protein[1], B0842[2][1], CmlA[2][1], MdfA[2][1], Cmr[2][1] , cmlA, cmr, ECK0832, JW0826, b0842 |
| Product description |
MdfA/MdfA MFS multidrug transporter[2][3] Transmembrane multidrug/chloramphenicol efflux transporter; confers alkaline pH tolerence[4] |
| EC number (for enzymes) |
|
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005215 |
transporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006810 |
transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016020 |
membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016021 |
integral to membrane |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0055085 |
transmembrane transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0055085 |
transmembrane transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
plasma membrane |
C-terminus localized in the cytoplasm with 12 predicted transmembrane domains |
Daley et al. (2005) [5] |
||
|
membrane |
| |||
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MQNKLASGAR LGRQALLFPL CLVLYEFSTY IGNDMIQPGM LAVVEQYQAG IDWVPTSMTA YLAGGMFLQW LLGPLSDRIG RRPVMLAGVV WFIVTCLAIL LAQNIEQFTL LRFLQGISLC FIGAVGYAAI QESFEEAVCI KITALMANVA LIAPLLGPLV GAAWIHVLPW EGMFVLFAAL AAISFFGLQR AMPETATRIG EKLSLKELGR DYKLVLKNGR FVAGALALGF VSLPLLAWIA QSPIIIITGE QLSSYEYGLL QVPIFGALIA GNLLLARLTS RRTVRSLIIM GGWPIMIGLL VAAAATVISS HAYLWMTAGL SIYAFGIGLA NAGLVRLTLF ASDMSKGTVS AAMGMLQMLI FTVGIEISKH AWLNGGNGLF NLFNLVNGIL WLSLMVIFLK DKQMGNSHEG |
| Length |
410 |
| Mol. Wt |
44.323 kDa |
| pI |
9.1 (calculated) |
| Extinction coefficient |
69,900 - 70,400 (calc based on 10 Y, 10 W, and 4 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
