lambda DE3: Difference between revisions
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m Created page with 'lambda DE3 is a prophage that expresses T7 RNA polymerase under the control of a lacUV5 promoter. == References == {{RefHelp}}<references/>' |
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lambda DE3 is a | lambda DE3 is a phage construct that expresses T7 RNA polymerase under the control of a lacUV5 promoter. | ||
Studier and Moffat<ref name='PMID:6708104'/> constructed lambda DE3 as one of a series of phage constructs where [[Phage_T7_1:Gene|T7 gene 1]] was cloned into the lambda D69<ref name='PMID:6299896'/> a unique BamH1. D69 is: | |||
Bam1<sup>0</sup> srI(1-2)delta att<sup>+</sup> imm21 nin5 shn6 | |||
In DE3, transcription of T7 gene 1 is rightward toward the int promoter. The BamH1 site where PlacUV5-T7 1 is inserted is in the int gene, so DE3 is an int mutant. Lysogens of DE3 are made using a helper phage of heterologous immunity. The int mutation should also prevent normal excision from lysogens. | |||
== References == | == References == | ||
{{RefHelp}}<references/> | {{RefHelp}}<references/> | ||
Latest revision as of 19:56, 8 October 2010
lambda DE3 is a phage construct that expresses T7 RNA polymerase under the control of a lacUV5 promoter.
Studier and Moffat[1] constructed lambda DE3 as one of a series of phage constructs where T7 gene 1 was cloned into the lambda D69[2] a unique BamH1. D69 is:
Bam10 srI(1-2)delta att+ imm21 nin5 shn6
In DE3, transcription of T7 gene 1 is rightward toward the int promoter. The BamH1 site where PlacUV5-T7 1 is inserted is in the int gene, so DE3 is an int mutant. Lysogens of DE3 are made using a helper phage of heterologous immunity. The int mutation should also prevent normal excision from lysogens.
References
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