pDG1i-eGFP-V5 blast
See Help:Plasmid for more information about plasmids in EcoliWiki.
At a Glance
<protect>
| Type |
|---|
| Origin of Replication |
| Copy # |
| Markers |
| Link to Sequence |
| Features |
| Accession Number |
</protect>
Notes
This is a lentiviral Gateway destination vector, with the tet-inducible minimal human CMV-IE promotor.
The plasmid is suitable for microRNA expression.
This vector can not be used to express short hairpin RNA (shRNA), which requires an RNA-polymerase III promotor. However, this vector can be used for knock-down experiments if the hairpin is expressed in a micro-RNA context (eg. Block-iT PolII miR RNAi kit).
The U3 region of the HIV-1 3' LTR is deleted (ΔU3 or dU3) and facilitates self-inactivation of the HIV-1 5' LTR after transduction to enhance the biosafety of the vector. The element also contains a polyadenylation signal for transcription termination and polyadenylation of mRNA in transduced cells.
The vector contains the psi packaging signal, allowing viral packaging, and the rev response element (RRE) from HIV-1, permitting Rev-dependent nuclear export of unspliced viral mRNA.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
You can help EcoliWiki by writing about the available sources of this plasmid. BCCM/LMBP Plasmid Collection
References
See Help:References for how to manage references in EcoliWiki. De Groote et al., BioTechniques 60 (2016), 252-259
Sequence
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.
actagtccagtgtggtggaattctgcagatatcaacaagtttgtacaaaaaagctgaacg agaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagac tacataatactgtaaaacacaacatatccagtcactatggcggccgcattaggcacccca ggctttacactttatgcttccggctcgtataatgtgtggattttgagttaggatccggcg agattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgtt gatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgt acctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaat aagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccg gaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgt tacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgac gatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctg gcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtg agtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttc accatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggtt catcatgccgtctgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtac tgcgatgagtggcagggcggggcgtaaagatctggatccggcttactaaaagccagataa cagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacc cgaagtatgtcaaaaagaggtgtgctatgaagcagcgtattacagtgacagttgacagcg acagctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaac catgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaagg gatggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggga ctggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttg tggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggcca gtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcgggg atgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcgggg aagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgt tctggggaatataaatgtcaggctccgttatacacagccagtctgcaggtcgaccatagt gactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaattt aatatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaagtggtt gatatccagcacagtggcggccgctcgagtatggtgagcaagggcgaggagctgttcacc ggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtg tccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcacc accggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcag tgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgccc gaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgc gccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgac ttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaac gtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccac aacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggc gacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaa gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatc actctcggcatggacgagctgtacaaggagggcccgcggttcgaaggtaagcctatccct aaccctctcctcggtctcgattctacgcgtaccggttagtaatgagtttggaattaattc tgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccaggcaggcagaagt atgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctcccca gcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgccccta actccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctga ctaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaag tagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtata tccattttcggatctgatcagcacgtgttgacaattaatcatcggcatagtatatcggca tagtataatacgacaaggtgaggaactaaaccatggccaagcctttgtctcaagaagaat ccaccctcattgaaagagcaacggctacaatcaacagcatccccatctctgaagactaca gcgtcgccagcgcagctctctctagcgacggccgcatcttcactggtgtcaatgtatatc attttactgggggaccttgtgcagaactcgtggtgctgggcactgctgctgctgcggcag ctggcaacctgacttgtatcgtcgcgatcggaaatgagaacaggggcatcttgagcccct gcggacggtgccgacaggtgcttctcgatctgcatcctgggatcaaagccatagtgaagg acagtgatggacagccgacggcagttgggattcgtgaattgctgccctctggttatgtgt gggagggctaagcacaattcgagctcggtacctttaagaccaatgacttacaaggcagct gtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaa cgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcc tgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttga gtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcaga cccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcag tatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagct tataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttca ctgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctag ctatcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctc cgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctg agctattccagaagtagtgaggaggcttttttggaggcctagggacgtacccaattcgcc ctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactggga aaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcg taatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcga atgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgt gaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttct cgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccg atttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtag tgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaa tagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttga tttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaa atttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcgggga aatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctc atgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtatt caacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgct cacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggt tacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgt tttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgac gccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtac tcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgct gccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccg aaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgg gaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagca atggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaa caattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggccctt ccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatc attgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacgggg agtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgatt aagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaactt catttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatc ccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatct tcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggc ttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccac ttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggct gctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggat aaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacg acctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaa gggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagg gagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctga cttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagc aacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcct gcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgct cgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgccca atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg tttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcat taggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagc ggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcgcaattaac cctcactaaagggaacaaaagctggagctgcaagcttaatgtagtcttatgcaatactct tgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagc accgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaac agacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtat ttaagtgcctagctcgatacataaacgggtctctctggttagaccagatctgagcctggg agctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgc ttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagaccct tttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagg gaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagagg cgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagag agatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaat tcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcag ggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagaca aatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattata taatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaagga agctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggc cgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatata aatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagag tggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggag cagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattat tgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatc tgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaa gatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgca ccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatc acacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactcct taattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagata aatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattat tcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatag tgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccga ggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagat ccattcgattagtgaacggatctcgacggtatcgatggtccgaggttctagacgagttta ctccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgta tgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtg atagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttact ccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataa gcagagctcgtttagtgaaccgtcagatcgcaccggtc
