See Help:Plasmid for more information about plasmids in EcoliWiki.
At a Glance
|Origin of Replication|
|Link to Sequence|
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The expression vector pBS(Kan) was constructed by removing the kanamycin resistance cartridge from pKG1022 by digestion with KpnI and BamHI, blunt-ending the fragments using T4 DNA polymerase, and then blunt-ligating into the ScaI site in the middle of the ampicillin resistance gene of pBluescript II KS- (Stratagene, La Jolla, Calif.).
You can help EcoliWiki by writing about the available sources of this plasmid.
See Help:References for how to manage references in EcoliWiki.
- Gerdes, K. (1988) The parB (hok/sok) locus of plasmid R1: a general purpose plasmid stabilization system. Nature Biotechnology 6:1402-1405
- Canada, KA et al. (2002) Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation. J. Bacteriol. 184 344-9 PubMed
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.