pBS(Kan)

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See Help:Plasmid for more information about plasmids in EcoliWiki.


At a Glance

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Type

Expression

Origin of Replication
Copy #
Markers
Link to Sequence
Features
Accession Number


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Notes

History

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The expression vector pBS(Kan) was constructed by removing the kanamycin resistance cartridge from pKG1022[1] by digestion with KpnI and BamHI, blunt-ending the fragments using T4 DNA polymerase, and then blunt-ligating into the ScaI site in the middle of the ampicillin resistance gene of pBluescript II KS- (Stratagene, La Jolla, Calif.).[2]

Sources

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References

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  1. Gerdes, K. (1988) The parB (hok/sok) locus of plasmid R1: a general purpose plasmid stabilization system. Nature Biotechnology 6:1402-1405
  2. Canada, KA et al. (2002) Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation. J. Bacteriol. 184 344-9 PubMed

Sequence

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