PMID:19706412
| Citation |
Peters, JM, Mooney, RA, Kuan, PF, Rowland, JL, Keles, S and Landick, R (2009) Rho directs widespread termination of intragenic and stable RNA transcription. Proc. Natl. Acad. Sci. U.S.A. 106:15406-11 |
|---|---|
| Abstract |
The transcription termination factor Rho is a global regulator of RNA polymerase (RNAP). Although individual Rho-dependent terminators have been studied extensively, less is known about the sites of RNAP regulation by Rho on a genome-wide scale. Using chromatin immunoprecipitation and microarrays (ChIP-chip), we examined changes in the distribution of Escherichia coli RNAP in response to the Rho-specific inhibitor bicyclomycin (BCM). We found approximately 200 Rho-terminated loci that were divided evenly into 2 classes: intergenic (at the ends of genes) and intragenic (within genes). The intergenic class contained noncoding RNAs such as small RNAs (sRNAs) and transfer RNAs (tRNAs), establishing a previously unappreciated role of Rho in termination of stable RNA synthesis. The intragenic class of terminators included a previously uncharacterized set of short antisense transcripts, as judged by a shift in the distribution of RNAP in BCM-treated cells that was opposite to the direction of the corresponding gene. These Rho-terminated antisense transcripts point to a role of noncoding transcription in E. coli gene regulation that may resemble the ubiquitous noncoding transcription recently found to play myriad roles in eukaryotic gene regulation. |
| Links |
PubMed PMC2741264 Online version:10.1073/pnas.0903846106 |
| Keywords |
Bicyclo Compounds, Heterocyclic; Chromatin Immunoprecipitation; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/metabolism; Genes, Bacterial/genetics; Microarray Analysis; RNA/biosynthesis; Transcription, Genetic/physiology; rho GTP-Binding Proteins/antagonists & inhibitors; rho GTP-Binding Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
Data
<protect>
| Strain/Organism | Assay | Analyte | Variable Conditions | Notes | Links |
|---|---|---|---|---|---|
|
MG1655 |
ChIP-chip |
RpoC RNAP beta prime |
RNAP in response to the Rho-specific inhibitor bicyclomycin (BCM) |
||
|
MG1655 |
ChIP-chip |
RpoB RNAP beta |
RNAP in response to the Rho-specific inhibitor bicyclomycin (BCM) |
||
|
MG1655 |
ChIP-chip |
RpoB RNAP beta |
Untreated |
||
|
MG1655 |
ChIP-chip |
RpoC RNAP beta prime |
Untreated |
||
| edit table |
</protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
