Cis Element:Plasmid cer

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Cis element summary table

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Standard Name

cer

Synonyms
Function

Plasmid Recombination Site

Location
SO terms


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Notes

Location and Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

ColE1

GGTGCGTACAATTAAGGGATTATGGTAAAT[1][2]

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Notes

Interactions

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Partner Type Partner Notes References Evidence

Protein

XerC

Protein

XerD

PMID:8605888[3]

Protein

ArgR

Protein

PepA

Protein

ArgR

may be indirect

PMID:8168483[4]

in vivo recombination assays

Protein

PepA

may be indirect

PMID:8168483[4]

in vivo recombination assays

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Notes

  • Xer-mediated recombination generates Holliday junction-containing DNA molecules[4].
  • The product of recombination at cer is a right-handed antiparallel catenane[5].

Mutant alleles and phenotypes

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Allele Nt change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

pSD115

Plasmid Clone

Reporter plasmid containing two directly repeated cer sites

PMID:8168483[4]

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Notes

Accessions

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Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

References

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  1. Blakely, G et al. (1991) Escherichia coli XerC recombinase is required for chromosomal segregation at cell division. New Biol. 3 789-98 PubMed
  2. Cornet, F et al. (1994) Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif. J. Bacteriol. 176 3188-95 PubMed
  3. 3.0 3.1 3.2 3.3 Colloms, SD et al. (1996) Xer-mediated site-specific recombination in vitro. EMBO J. 15 1172-81 PubMed
  4. 4.0 4.1 4.2 4.3 McCulloch, R et al. (1994) Xer-mediated site-specific recombination at cer generates Holliday junctions in vivo. EMBO J. 13 1844-55 PubMed
  5. Colloms, SD et al. (1997) Topological selectivity in Xer site-specific recombination. Cell 88 855-64 PubMed

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