E. coli Genome Project at UW-Madison

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E. coli Genome Project

What:

Annoations of the E. coli K-12 genome and its annotations. Creating knockouts of every gene, clone of every ORF, and extensive gene expression.

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About E. coli Genome Project at UW-Madison

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Content

Genomic sequence data of various organisms and their annotation. There are several resources including:

  • Sequencing Projects

E.coli K12 MG1655
E.coli )157:H7
Uropathogenic E.coli
E.coli K1 (NMEC)
Shigella flexneri 2a
Salmonella typhi
Yersinia pestis

Plasmids
Other Enterobacterial Genome Projects See list

  • Functional Genomics

Archive Cloning with each ORF cloned in a standard vector
Systematic Mutagenesis: A strain collection consisting of a mutant in every gene
DNA Microarray experiments of wt and nutants inder different conditions
Phenotypic analysis of mutants
Protocols and Recipes
List of some essential genes in E. coli

  • BLAST & ASAP Database information

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Discussion

References

M. Liu, T. Durfee, J. E. Cabrera, K. Zhao, D. J. Jin, and F. R. Blattner (2005) Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli. J Biol Chem 280(16): 15921-15927 PubMed

External Links

E. coli Genome Project at UW-Madison

Discussion of E. coli Genome Project at UW-Madison on other websites