E. coli Genome Project at UW-Madison
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| What: |
Annotations of the E. coli K-12 genome and its annotations. Creating knockouts of every gene, clone of every ORF, and extensive gene expression under varying physiological conditions. |
| Who: |
University of Wisconsin - Madison |
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About E. coli Genome Project at UW-Madison
Add general information here E. coli Genome Project at UW-Madison is...
Content
Genomic sequence data of various organisms and their annotation. There are several resources including:
- Sequencing Projects
E.coli K-12 MG1655
E.coli )157:H7
Uropathogenic E.coli
E.coli K1 (NMEC)
Shigella flexneri 2a
Salmonella typhi
Yersinia pestis
Plasmids
Other Enterobacterial Genome Projects See list
- Functional Genomics
- Functional Genomics
Archive Cloning with each ORF cloned in a standard vector
Systematic Mutagenesis: A strain collection consisting of a mutant in every gene
DNA Microarray experiments of wt and nutants inder different conditions
Phenotypic analysis of mutants
Protocols and Recipes
List of some essential genes in E. coli
- BLAST & ASAP Database information
Using E. coli Genome Project at UW-Madison
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Technology
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Discussion
References
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M. Liu, T. Durfee, J. E. Cabrera, K. Zhao, D. J. Jin, and F. R. Blattner (2005) Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli. J Biol Chem 280(16): 15921-15927 PubMed
External Links
E. coli Genome Project at UW-Madison
