BW25113

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Alternate Names

Chromosomal Genotype

∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda^-, rph-1, ∆(rhaD-rhaB)568, hsdR514

Plasmids

Phage

Phenotypes

Construction

Notes

BW25113 was used to make the Keio Collection of single-gene knockouts. ^[1] ^[2] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. ^[2] said the strain is lacI^Q, but it was later shown to be lacI⁺ (Supplementary Table 1)^[1].

References

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↑ ^1.0 ^1.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
↑ ^2.0 ^2.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed

[PMID:16738554-1] 1.0 ^1.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed

[PMID:10829079-2] 2.0 ^2.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed

[1]

[2]