pGEX-4T-3

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At a Glance

[[Image:#PGEX.jpg|right|thumb|600px|pGEX-4T-3; Image provided by GE Healthcare]]

Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

PubMed

Features

tac Promoter
lac Operator
RBS
MCS
lacIq

Accession Number

U13855

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Notes

pGEX-4T-3 is derived from pGEX-2T and contains a thrombin recognition site. pGEX-4T-3 allows for removal of the GST carrier protein from the fusion protein by enzymatic cleavage with thrombin.

History

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Sources

GE Healthcare

References

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Sequence

You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.