pGEX-4T-3

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At a Glance

pGEX-4T-3, Image made with PlasMapper

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Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

tac Promoter
lac Operator
RBS
MCS
lacIq

Accession Number

U13855

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Notes

pGEX-4T-3 is derived from pGEX-2T and contains a thrombin recognition site. pGEX-4T-3 allows for removal of the GST carrier protein from the fusion protein by enzymatic cleavage with thrombin.

History

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Sources

GE Healthcare

References

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Sequence

       1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
      61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     901 atcctccaaa atcggatctg gttccgcgtg gatccccgaa ttcccgggtc gactcgagcg
     961 gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
    1021 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    1081 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
    1141 gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct
    1201 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    1261 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    1321 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    1381 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    1441 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    1501 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    1561 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
    1621 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    1681 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    1741 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    1801 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    1861 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
    1921 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    1981 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    2041 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    2101 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    2161 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    2221 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    2281 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    2341 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    2401 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    2461 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    2521 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
    2581 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    2641 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    2701 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    2761 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    2821 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    2881 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    2941 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    3001 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    3061 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    3121 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    3181 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taaattccga
    3241 caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
    3301 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
    3361 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
    3421 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
    3481 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
    3541 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
    3601 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
    3661 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
    3721 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
    3781 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
    3841 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
    3901 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
    3961 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
    4021 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
    4081 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
    4141 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
    4201 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
    4261 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
    4321 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    4381 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    4441 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    4501 aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt cgttttacaa
    4561 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    4621 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    4681 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
    4741 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
    4801 cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg
    4861 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
    4921 tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt