pCC1BAC

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At a Glance

Type

Cloning

Origin of Replication

single-copy F-factor, multi-copy oriV

Copy #

single copy and can be induced to 10-20 copies per cell

Markers
Link to Sequence

PubMed

Comments

Copy Control Vector; For oriV to function as an origin of replication, oriV requires a gene product called "trfA". Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter. Each Cloning Ready pCC1BAC Vector has been linearized at a unique BamH 1, EcoR 1 or Hind III site.

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History

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Sources

Epicentre

References

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Sequence

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