pCC1BAC
At a Glance
pCC1BAC, Image made with PlasMapper
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| Type |
Cloning |
|---|---|
| Origin of Replication |
single-copy F-factor, multi-copy oriV |
| Copy # |
single copy and can be induced to 10-20 copies per cell |
| Markers | |
| Link to Sequence | |
| Features |
Copy Control BamH I, EcoR I, or Hind III Cloning Ready pCC1BAC Vectors; For oriV to function as an origin of replication, oriV requires a gene product called "trfA". Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter. |
| Accession Number | |
| edit table |
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Notes
History
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Sources
References
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Sequence
1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccacacagat
121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatcctc
361 tagagtcgac ctgcaggcat gcaagcttga gtattctata gtctcaccta aatagcttgg
421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
601 attaatgaat cggccaacgc gaaccccttg cggccgcccg ggccgtcgac caattctcat
661 gtttgacagc ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg
721 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
961 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
1501 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
1561 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
1621 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
1681 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
1741 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
1801 ttcctatgcg atgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
1861 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
1921 tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat gcggttgcga tgctgaaaca
1981 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
2041 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
2101 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
2161 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
2221 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
2281 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
2341 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcgagctggt tgccctcgcc
2401 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
2461 gcgagacacc gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac
2521 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
2581 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
2641 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
2701 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
2761 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
2821 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
2881 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
2941 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
3001 ggtcgagtga gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg
3061 cctgaaaaaa cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt
3121 tttttatagt ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt
3181 tctagagaag gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca
3241 gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt
3301 ttttcacaaa gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac
3361 ttgtcacact tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg
3421 taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc
3481 ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac
3541 cctacaggaa catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg
3601 attgacctct gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa
3661 ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt
3721 tccttggttt atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa
3781 cccatatctc attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag
3841 tgaaacaaaa gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta
3901 tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta
3961 ccagctgcct caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg
4021 tgttaatgag atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg
4081 ccgccagacg actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata
4141 gtctgagggt tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg
4201 agggtaattt gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt
4261 tgaactgtaa tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc
4321 tctttccctt cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt
4381 gattatcaca gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt
4441 ttcccacggt ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct
4501 tctttgcttc ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag
4561 tgataataag tgactgaggt atgtgctctt cttatctcct tttgtagtgt tgctcttatt
4621 ttaaacaact ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt
4681 gcaagattta ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg
4741 gaaacactta accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca
4801 cagtttaatg atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa
4861 gcagcggatt tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga
4921 ctaccgcacc cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt
4981 gaacaaatta atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta
5041 tttccaccgg tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct
5101 gttcatcttg ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac
5161 gacccccagg gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca
5221 gaagacactc tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag
5281 cccacttgct ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa
5341 actgagttaa tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc
5401 cgactggcca ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct
5461 aacctgggta tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg
5521 cctgctgagt tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg
5581 ctcaagaacg ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac
5641 agcaatagca atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga
5701 agcatggttc taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg
5761 atgagaactg tttttgaaca ggccattgat caacgctctt caactggtgc ctggagaaat
5821 gctctttcta tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc
5881 tgggagatta gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac
5941 cggttgaaga tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc
6001 gcgtaggagt aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga
6061 agtttactct tgaagtgctc cggggtgata gtgttgagaa gacctctcgg gtatggtcag
6121 gtaatgaacg tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt
6181 ttctactgac tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa
6241 ttgccgatgg gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc
6301 tggttggcga gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc
6361 gcccaacaag tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg
6421 ctggaaatat ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct
6481 gtatcaacac cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac
6541 tatctgcccg gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta
6601 agcagcaggc atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag
6661 aagttatcac tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa
6721 gctcacgaca tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc
6781 ttaacctgga caggtctcgt gttccaactg agtgtataga gaaaattgag gccattctta
6841 aggaacttga aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt
6901 tacttaatgt cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc
6961 ccactgttcc acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta
7021 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
7081 agtctgggac cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc
7141 ccactcgtat cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt
7201 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga
7261 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
7321 atcgtcggtc tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat
7381 cggtctggga ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat
7441 tccatcaatg cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta
7501 acctcggtgt gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac
7561 attttgcgca cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa
7621 ccgggctgca tccgatgcaa gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg
7681 ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga
7741 tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc
7801 tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc ctttccggtg
7861 atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg
7921 tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa ataccctctg
7981 aaaagaaagg aaacgacagg tgctgaaagc gagctttttg gcctctgtcg tttcctttct
8041 ctgtttttgt ccgtggaatg aacaatggaa gtccgagctc atcgctaata acttcgtata
8101 gcatacatta tacgaagtta tattcgat
