XL-2 Blue

Sources

Stratagene

Chromosomal Genotype

endA1 supE44 thi-1 recA1 gyrA96 relA1 lac

Phage

F´ proAB lacIqZΔM15 Tn10 (Tetr)

Phenotypes

Tetracycline resistant

Construction

Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 Amy Camr]. (Genes listed signify mutant alleles. Genes on the F´ episome, however, are wild-type unless indicated otherwise.)

Notes

The XL-2 Blue MRF' (Minus Restriction) strain is deficient in all known restriction systems (McrA-, McrCB-, McrF-, Mrr-, HsdR-), preventing cleavage of cloned DNA that carries cytosine and/or adenine methylation, which is often present in eukaryotic DNA and cDNA.(Stratagene)

References

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