Nonsense suppressor

Nonsense suppressors change the effects of nonsense mutations by altering how stop codons are read. The largest class of nonsense suppressors are mutations in Category:tRNA genes that alter the codon-anticodon interaction to allow a stop codon to be read by that tRNA. This will only suppress the mutation if the amino acid inserted restores a functional protein product.

Nonsense suppression is defined genetically based on reversing an observable phenotype. Note that this does not mean full restoration of normal function. Nonsense suppression is often incomplete ("leaky") and pleiotropic.

gene	synonyms	tRNA affected	aa inserted	codons recognized
serU, serV	supD, supH, su1, Su-1, SuI, ftsM, su_1	tRNA^Ser(CGA) (Serine tRNA2)	Serine ^[1]	Amber
glnX, glnV	supE, Su2, Su_II	tRNA^Gln(CUG) (Glutamine tRNA2)	Glutamine^[2]	Amber
tyrT	supF	tRNA^Tyr(GUA) (Tyrosine tRNA1)	Tyrosine^[2]	Amber
lysT	supG, supK, supL, Su(beta), su-5,	tRNA^Lys(UUU) (Lysine tRNA)	Lysine	Ochre
leuX	supP, Su-6	tRNA^Leu(CAA) (Leucine tRNA5)	Leucine	Amber
tyrT	supC	tRNA^Tyr(GUA) (Tyrosine tRNA1)	Tyrosine^[2]	Ochre
trpT	supU, supV, su9	tRNA^Trp(CCA) (Tryptophan tRNA)	Tryptophan	Opal
tyrU	supZ	tRNA^Tyr(GUA) (Tyrosine tRNA2)	Tyrosine	Amber
glnU	supB	tRNA^Gln(UUG) (Glutamine tRNA1)	Glutamine	Ochre
lysT	supL	tRNA^Lys(UUU) (Lysine tRNA)	Lysine	Ochre
edit table

This table is incomplete and may have inaccuracies in the synonym lists for amber vs ochre. Need to add the engineered suppressors from Kleina et al^[3] and Normanly et al^[4]. Others?

tRNA suppressors

non-tRNA nonsense suppressors

References

↑ WEIGERT, MG & GAREN, A (1965) AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE. J. Mol. Biol. 12 448-55 PubMed
↑ ^2.0 ^2.1 ^2.2 Weigert, MG et al. (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene. J. Mol. Biol. 14 522-7 PubMed
↑ Kleina, LG et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency. J. Mol. Biol. 213 705-17 PubMed
↑ Normanly, J et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J. Mol. Biol. 213 719-26 PubMed

[PMID:14337506-1] WEIGERT, MG & GAREN, A (1965) AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE. J. Mol. Biol. 12 448-55 PubMed

[PMID:4286570-2] 2.0 ^2.1 ^2.2 Weigert, MG et al. (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene. J. Mol. Biol. 14 522-7 PubMed

[PMID:_2193162-3] Kleina, LG et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency. J. Mol. Biol. 213 705-17 PubMed

[PMID:_2141650-4] Normanly, J et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J. Mol. Biol. 213 719-26 PubMed

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Nonsense suppressor

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tRNA suppressors

non-tRNA nonsense suppressors

See Also

References

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Nonsense suppressor

tRNA suppressors

non-tRNA nonsense suppressors

See Also

References

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