Nonsense suppressor
Nonsense suppressors change the effects of nonsense mutations by altering how stop codons are read. The largest class of nonsense suppressors are mutations in Category:tRNA genes that alter the codon-anticodon interaction to allow a stop codon to be read by that tRNA. This will only suppress the mutation if the amino acid inserted restores a functional protein product.
| gene | synonyms | tRNA affected | aa inserted | codons recognized |
|---|---|---|---|---|
|
supD, supH, su1, Su-1, SuI, ftsM, su_1 |
Serine [1] |
Amber | ||
|
supE, Su2, Su_II |
Glutamine[2] |
Amber | ||
|
supF |
Tyrosine[2] |
Amber | ||
|
supG, supK, supL, Su(beta), su-5, |
Lysine |
Ochre | ||
|
supP, Su-6 |
Leucine |
Amber | ||
|
supC |
Tyrosine[2] |
Ochre | ||
|
supU, supV, su9 |
Tryptophan |
Opal | ||
|
supZ |
Tyrosine |
Amber | ||
|
supB |
Glutamine |
Ochre | ||
|
supL |
Lysine |
Ochre | ||
| edit table |
This table is incomplete and may have inaccuracies in the synonym lists for amber vs ochre. Need to add the engineered suppressors from Kleina et al[3] and Normanly et al[4]. Others?
See Also
- http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/rev-sup/nonsense-suppressors.html Stan Maloy's page on nonsense suppressors
References
- ↑ WEIGERT, MG & GAREN, A (1965) AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE. J. Mol. Biol. 12 448-55 PubMed
- ↑ 2.0 2.1 2.2 Weigert, MG et al. (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene. J. Mol. Biol. 14 522-7 PubMed
- ↑ Kleina, LG et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency. J. Mol. Biol. 213 705-17 PubMed
- ↑ Normanly, J et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J. Mol. Biol. 213 719-26 PubMed