MG1655 seqA-eYFP

Name	MG1655 seqA-eYFP
Synonyms
Genotype	F– λ– ilvG– rfb-50 rph-1 seqA-eYFP:kmR
Phenotype	Kanamycin resistant
Sources	LMBP 9776
References	PMID:26449690

Notes

The native seqA gene was fused at its 3' end to the fluorescent protein gene eYFP, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.