MG1655 seqA-eYFP
Name | MG1655 seqA-eYFP |
Synonyms | |
Genotype | F– λ– ilvG– rfb-50 rph-1 seqA-eYFP:kmR |
Phenotype | Kanamycin resistant |
Sources | LMBP 9776 |
References | PMID:26449690 |
Notes
The native seqA gene was fused at its 3' end to the fluorescent protein gene eYFP, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome.
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.