pBAD24

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See Help:Plasmid for more information about plasmids in EcoliWiki.


At a Glance

Type

Cloning Expression Vector

Origin of Replication
  • pBR322/ColE1
  • M13
Copy #
Markers

AmpR

Link to Sequence

GenBank:X81837.1

Features
  • araC
  • optimized SD sequence
  • Kozak sequence (for efficient translation in eukaryotic cells)[1]
  • translation start codon
Accession Number

GenBank:X81837.1


Notes

  • used to clone gene which lack sequences for initiation of translation[2]

History

Construction described in Guzman et al. (1995)[2].

Sources

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References

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  1. ↑ Kozak, M (1987) An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15 8125-48 PubMed
  2. ↑ 2.0 2.1 Guzman, LM et al. (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177 4121-30 PubMed

Sequence

You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.