PMID:7028742

Newman, MJ, Foster, DL, Wilson, TH and Kaback, HR (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli.J. Biol. Chem. 256:11804-8

Abstract

<protect>The lactose carrier protein of Escherichia coli was purified by a simple procedure employing differential solubilization and ion-exchange chromatography and reconstituted into liposomes by octylglucoside dilution. The proteoliposomes exhibited both membrane potential-driven lactose transport and lactose counterflow. Furthermore, the purified protein was identified as the product of the lac y gene. These and other results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow.

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