DNA Mutagenesis

About

DNA mutagenesis introduces mutations into the DNA sequence as a way to generate mutants to study protein function. Chemical mutagenesis is one method these mutations may be introduced.

Types of Chemical Mutagens

• Hydroxylamine (HA)

HA causes C->T and G->A transitions in the DNA

• Formic Acid (FA)

FA causes G->A and A->G transitions as well as G->T, A->T, G->C, and A->C transversions in the DNA

• Hydrazine (HZ)

HZ induces C->T and G->A transitions