DH10B

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Alternate Names

The sequence of DH10B was published in 2008[1] .

DH10B

Sources

Chromosomal Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
From J Jessee.[2]

Plasmids

not specified

Phage

not specified

Phenotypes

Construction

unknown parents.

Comments

host for pUC and other α-complementation vectors; pBR322
useful for generating genomic libraries containing methylated cytosine or adenine residues
useful for plasmid rescue procedures
Copyed from GIBCO

References

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  1. ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  2. ↑ Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed