pF4A CMV: Difference between revisions
Jump to navigation
Jump to search
edited by Pei via TableEdit |
|||
| Line 32: | Line 32: | ||
!{{table heading style}} |Features | !{{table heading style}} |Features | ||
| | | | ||
CMV <br> T7 Promoter <br> | CMV enhancer/promoter <br> T7 Promoter <br> Sgf I site <br> Barnase coding region <br> Pme I site <br> Beta lactamase (Ampr) coding region <br> cer site | ||
|- | |- | ||
!{{table heading style}} |Accession Number | !{{table heading style}} |Accession Number | ||
Revision as of 06:05, 5 March 2008
At a Glance
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
CMV enhancer/promoter |
| Accession Number | |
| edit table | |
Notes
pF4A and pF4K CMV Flexi® Vectors(a,b,f): These vectors are designed for constitutive native (untagged) protein expression in mammalian cells. The vectors provide constitutive protein expression in mammalian cells using the human cytomegalovirus (CMV) intermediate-early enhancer/promoter. The vectors also contain the T7 RNA polymerase promoter for in vitro protein expression. These vectors can be used for both stable and transient gene expression, but for stable expression, cotransfection with a vector containing a selectable marker is required. The vectors are available with either ampicillin (pF4A CMV Flexi® Vector) or kanamycin resistance (pF4K CMV Flexi® Vector) for selection in E. coli.
constitutive expression of native protein
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA
51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAAGG AATCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCA GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAA CGAATTCGAG CTCGGTACCC 1451 GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT CCGGCTGCTA 1501 ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA 1551 CTAGCATAAC CCCTTGGGGC GGCCGCTTCG AGCAGACATG ATAAGATACA 1601 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT 1651 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 1701 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 1751 AGGGGGAGAT GTGGGAGGTT TTTTTAAGCA AGTAAAACCT CTACAAATGT 1801 GGTAAAATCG AATTCTAATG GATCCTCTTT GCGCTTGCGT TTTCCCTTGT 1851 CCAGATAGCC CAGTAGCTGA CATTCATCCG GGGTCAGCAC CGTTTCTGCG 1901 GACTGGCTTT CTACGTAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 1951 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 2001 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT 2051 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 2101 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT 2151 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 2201 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 2251 CGTTTTCCAA TGATGAGCAC TTTCAAAGTT CTGCTATGTG GCGCGGTATT 2301 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT 2351 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 2401 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA 2451 TAACACTGCG GCCAACTTAC TTCTGACAAC TATCGGAGGA CCGAAGGAGC 2501 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 2551 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 2601 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC 2651 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 2701 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT 2751 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 2801 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 2851 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 2901 TGCCTCACTG ATTAAGCATT GGTAATTCGA AATGACCGAC CAAGCGACGC 2951 CCAACCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 3001 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 3051 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 3101 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 3151 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 3201 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 3251 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 3301 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 3351 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 3401 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 3451 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 3501 CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 3551 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 3601 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 3651 CGCAGAAAAA AAGGATTTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 3701 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 3751 TATCAAAAAG GATCTTCACC TAGATCCTTT TATAGTCCGG AAATACAGGA 3801 ACGCACGCTG GATGGCCCTT CGCTGGGATG GTGAAACCAT GAAAAATGGC 3851 AGCTTCAGTG GATTAAGTGG GGGTAATGTG GCCTGTACCC TCTGGTTGCA 3901 TAGGTATTCA TACGGTTAAA ATTTATCAGG CGCGATTGCG GCAGTTTTTC 3951 GGGTGGTTTG TTGCCATTTT TACCTGTCTG CTGCCGTGAT CGCGCTGAAC 4001 GCGTTTTAGC GGTGCGTACA ATTAAGGGAT TATGGTAAAT CCACTTACTG 4051 TCTGCCCTCG TAGCCATCGA GATAAACCGC AGTACTCCGG CCACGATGCG 4101 TCCGGCGTAG AGGATCGAGA TCT