pBACgus-2cp

At a Glance

Type	Transfer
Origin of Replication	f1
Copy #	high
Markers	Ampicillin
Link to Sequence
Features	His Tag S Tag polh promoter region polh transcription start wt polh 5'UTR –1 position Multiple cloning sites (Sma I - Xho I gus gene encoding b-glucuronidase protein promoter (P6.9)
Accession Number
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History

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Sources

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References

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Sequence

       1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 
      61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 
     121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 
     181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 
     241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 
     301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 
     361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 
     421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 
     481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 
     541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 
     601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 
     661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 
     721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 
     781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 
     841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 
     901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 
     961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 
    1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 
    1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 
    1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg 
    1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg 
    1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa 
    1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga 
    1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc 
    1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc 
    1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg 
    1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg 
    1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta 
    1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt 
    1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct 
    1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt 
    1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca 
    1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg 
    1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg 
    2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca 
    2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact 
    2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg 
    2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa 
    2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg 
    2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg 
    2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg 
    2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg 
    2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca 
    2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat 
    2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa 
    2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag 
    2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga 
    2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa 
    2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt 
    2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta 
    3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa 
    3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc 
    3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata 
    3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg 
    3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat 
    3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa 
    3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat gcccatggtc 
    3421 catcatcacc accatcactc cgcgggtttg gtgcccagag gcagcggcaa agaaacggcg 
    3481 gcggcgaaat ttgaacgcca acacatggac agcgctagcg gtggaggtga tgacgacgac 
    3541 aagagcccgg gcttctcctc aaaaggcctg gatccgaatt cgagctcgaa gcttgcggcc 
    3601 gcactcgagc accaccacca ccaccactaa cctaggtagc tgagcgcatg caagctgatc 
    3661 cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt 
    3721 gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga 
    3781 aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt 
    3841 tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac 
    3901 tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag 
    3961 ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat 
    4021 tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca 
    4081 cgttaaatag agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat 
    4141 cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca 
    4201 cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt 
    4261 ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt 
    4321 ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca 
    4381 aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag 
    4441 gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag 
    4501 gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg 
    4561 ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca 
    4621 acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag 
    4681 cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca 
    4741 cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc 
    4801 gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc 
    4861 gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat 
    4921 ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa 
    4981 tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata 
    5041 acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgagg 
    5101 tttaaacgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
    5161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
    5221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
    5281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
    5341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
    5401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
    5461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
    5521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
    5581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
    5641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
    5701 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
    5761 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
    5821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
    5881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
    5941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
    6001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
    6061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
    6121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
    6181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
    6241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
    6301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
    6361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
    6421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
    6481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
    6541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
    6601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
    6661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
    6721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
    6781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
    6841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
    6901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
    6961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
    7021 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
    7081 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
    7141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
    7201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 
    7261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
    7321 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 
    7381 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
    7441 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc 
    7501 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca