pBACgus-2cp
At a Glance
Image of pBACgus-2cp. Novagen
<protect>
| Type |
Transfer |
|---|---|
| Origin of Replication |
f1 |
| Copy # |
high |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
His Tag |
| Accession Number |
|
| edit table |
</protect>
Notes
pBACgus-2cp is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter.The Stu I site in pBAC-2cp and pBACgus-2cp overlaps an E. coli dcm methylation site, which inhibits digestion when plasmids are produced in dcm+ hosts. These plasmids must be grown in dcm– hosts for the Stu I site to be used.
History
pBACgus-2cp is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat gcccatggtc
3421 catcatcacc accatcactc cgcgggtttg gtgcccagag gcagcggcaa agaaacggcg
3481 gcggcgaaat ttgaacgcca acacatggac agcgctagcg gtggaggtga tgacgacgac
3541 aagagcccgg gcttctcctc aaaaggcctg gatccgaatt cgagctcgaa gcttgcggcc
3601 gcactcgagc accaccacca ccaccactaa cctaggtagc tgagcgcatg caagctgatc
3661 cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt
3721 gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga
3781 aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt
3841 tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac
3901 tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag
3961 ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat
4021 tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca
4081 cgttaaatag agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat
4141 cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca
4201 cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt
4261 ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt
4321 ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca
4381 aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag
4441 gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag
4501 gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg
4561 ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca
4621 acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag
4681 cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca
4741 cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc
4801 gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc
4861 gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat
4921 ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa
4981 tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata
5041 acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgagg
5101 tttaaacgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
5161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
5221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
5281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
5341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
5401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
5461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
5521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
5581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
5641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
5701 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
5761 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
5821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
5881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
5941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
6001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
6061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
6121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
6181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
6241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
6301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
6361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
6421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
6481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
6541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
6601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
6661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
6721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
6781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
6841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
6901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
6961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
7021 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
7081 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
7141 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
7201 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
7261 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
7321 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
7381 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
7441 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc
7501 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
