pGPS1.1

At a Glance

Type
Origin
Copy #
Markers
Link to Sequence	http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pgps1.1.txt
Comments
References
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History

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Sources

pGPS1.1 is isolated from E. coli BW23474, an E. coli strain that expresses the pir-116 replication factor (4), by a standard plasmid purification procedure.

Sequence

      1  GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG 

     51  TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC 

    101  CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG 

    151  GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 

    201  CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC 

    251  ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT 

    301  CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA 

    351  GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT 

    401  AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA 

    451  CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC 

    501  ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA 

    551  GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG 

    601  TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA 

    651  CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA 

    701  AAGAGGTAAT GAAATGGCAG ATCCCTGGCT TGTTGTCCAC AACCGTTAAA 

    751  CCTTAAAAGC TTTAAAAGCC TTATATATTC TTTTTTTTCT TATAAAACTT 

    801  AAAACCTTAG AGGCTATTTA AGTTGCTGAT TTATATTAAT TTTATTGTTC 

    851  AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA CGTTAGCCAT 

    901  GAGAGCTTAG TACGTTAGCC ATGAGGGTTT AGTTCGTTAA ACATGAGAGC 

    951  TTAGTACGTT AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA 

   1001  CGTACTATCA ACAGGTTGAA CTGCTGATCT TCGGATCTAT GTCGGGTGCG 

   1051  GAGAAAGAGG TAATGAAATG GCATCCGGAT CTGCATCGCA GGATGCTGCT 

   1101  GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCA TTATTGAAGC 

   1151  ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 

   1201  GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 

   1251  CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG 

   1301  CGTATCACGA GGCCCTTTCG TCTTCAAGAA TTCTCATGTT TGACAGCTTA 

   1351  TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC 

   1401  AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG 

   1451  CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC 

   1501  CGGGCCTCTT GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT 

   1551  GGCGTGCTGC TAGCGCTATA TGCGTTGATG CAATTTCTAT GCGCACCCGT 

   1601  TCTCGGAGCA CTGTCCGACC GCTTTGGCCG CCGCCCAGTC CTGCTCGCTT 

   1651  CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC CACACCCGTC 

   1701  CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC 

   1751  AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC 

   1801  GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG 

   1851  GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC 

   1901  ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT 

   1951  TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT GCCCTTGAGA 

   2001  GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT 

   2051  CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC 

   2101  CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG 

   2151  ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC 

   2201  TCAAGCCTTC GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG 

   2251  CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG 

   2301  TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC 

   2351  CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG 

   2401  ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC 

   2451  CTAACTTCGA TCATTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC 

   2501  GGCGAGCACA TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC 

   2551  TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG 

   2601  ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA 

   2651  ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC 

   2701  TTGGCAGAAC ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC 

   2751  GCATCTCGGG CAGCGTTGGG TCCTGGGCTG GCATTGACCC TGAGTGATTT 

   2801  TTCTCTGGTC CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT 

   2851  TCCAGTAACC GGGCATGTTC ATCATCAGTA ACCCGTATCG TGAGCATCCT 

   2901  CTCTCGTTTC ATCGGTATCA TTACCCCCAT GAACAGAAAT CCCCCTTACA 

   2951  CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA CATGGCCCGC 

   3001  TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA 

   3051  CGCGGATGAA CAGGCAGAGC TCTTACTGTC ATGCCATCCG TATGTGGGCG 

   3101  GACAATAAAG TCTTAAACTG AACAAAATAG ATCTAAACTA TGACAATAAA 

   3151  GTCTTAAACT AGACAGAATA GTTGTAAACT GAAATCAGTC CAGTTATGCT 

   3201  GTGAAAAAGC ATACTGGACT TTTGTTATGG CTAAAGCAAA CTCTTCATTT 

   3251  TCTGAAGTGC AAATTGCCCG TCGTATTAAA GAGGGGCGTG GGGTCGACGC 

   3301  GGCCGCTAAC TATAACGGTC CTAAGGTAGC GAGTTTAAAC GATATCGGAT 

   3351  CCGGCCGCCG CTGAGGTCTG CCTCGTGAAG AAGGTGTTGC TGACTCATAC 

   3401  CAGGCCTGAA TCGCCCCATC ATCCAGCCAG AAAGTGAGGG AGCCACGGTT 

   3451  GATGAGAGCT TTGTTGTAGG TGGACCAGTT GGTGATTTTG AACTTTTGCT 

   3501  TTGCCACGGA ACGGTCTGCG TTGTCGGGAA GATGCGTGAT CTGATCCTTC 

   3551  AACTCAGCAA GAGTTCGATT TATTCAACAA AGCCGCCGTC CCGTCAAGTC 

   3601  AGCGTAATGC TCTGCCAGTG TTACAACCAA TTAACCAATT CTGATTAGAA 

   3651  AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT 

   3701  CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA 

   3751  CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC 

   3801  GACTCGTCCA ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA 

   3851  GGTTATCAAG TGAGAAATCA CCATGAGTGA CGACTGAATC CGGTGAGAAT 

   3901  GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG GCCAGCCATT 

   3951  ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG 

   4001  ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA 

   4051  CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC 

   4101  AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT 

   4151  TCCCGGGGAT CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA 

   4201  AAATGCTTGA TGGTCGGAAG AGGCATAAAT TCCGTCAGCC AGTTTAGTCT 

   4251  GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG CCATGTTTCA 

   4301  GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAATCGATA GATTGTCGCA 

   4351  CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC 

   4401  ATCCATGTTG GAATTTAATC GCGGCCTCGA GCAAGACGTT TCCCGTTGAA 

   4451  TATGGCTCAT AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT 

   4501  ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT 

   4551  TTGAGACACA ACGTGGCTTA CTAGGATCCG ATATCATTTA AATCTAGGGA 

   4601  TAACAGGGTA ATACTAGTGT CGACCAACCA GATAAGTGAA ATCTAGTTCC 

   4651  AAACTATTTT GTCATTTTTA ATTTTCGTAT TAGCTTACGA CGCTACACCC 

   4701  AGTTCCCATC TATTTTGTCA CTCTTCCCTA AATAATCCTT AAAAACTCCA 

   4751  TTTCCACCCC TCCCAGTTCC CAACTATTTT GTCCGCCCAC ACCGTAAGAT 

   4801  GCTTTTCTGT GACT

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References

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