pGPS1.1
At a Glance
pGPS1.1, Image provided by NEB
<protect>
| Type |
Transposon Donor |
|---|---|
| Origin of Replication |
R6K-γ[1] |
| Copy # |
High |
| Markers |
Tetracycline |
| Link to Sequence | |
| Features |
Transprimer-1 |
| Accession Number |
|
| edit table |
</protect>
Notes
pGPS1.1 is used as a trasposon donor in the GPS-1 Genome Priming System offered by NEB. TnsABC transposase removes the Tn7-based Transprimer element from pGPS1.1 and inserts it randomly into a trarget DNA molecule in vitro. pGPS1.1 is isolated from E. coli BW 23474, a strain that expresses the pir-116 replication factor (4).
History
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Sources
Sequence
1 GGTACCCTGT GAATGCGCAA ACCAACCCTT GGCAGAACAT ATCCATCGCG
51 TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA GCGTTGGGTC
101 CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG
151 GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
201 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC
251 ATGAATGGTC TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT
301 CAGCGCCCTG CACCATTATG TTCCGGATCT ATGTCGGGTG CGGAGAAAGA
351 GGTAATGAAA TGGCAGATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT
401 AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA
451 CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC
501 ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA
551 GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG
601 TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA
651 CTATCAACAG GTTGAACTGC TGATCTTCGG ATCTATGTCG GGTGCGGAGA
701 AAGAGGTAAT GAAATGGCAG ATCCCTGGCT TGTTGTCCAC AACCGTTAAA
751 CCTTAAAAGC TTTAAAAGCC TTATATATTC TTTTTTTTCT TATAAAACTT
801 AAAACCTTAG AGGCTATTTA AGTTGCTGAT TTATATTAAT TTTATTGTTC
851 AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA CGTTAGCCAT
901 GAGAGCTTAG TACGTTAGCC ATGAGGGTTT AGTTCGTTAA ACATGAGAGC
951 TTAGTACGTT AAACATGAGA GCTTAGTACG TGAAACATGA GAGCTTAGTA
1001 CGTACTATCA ACAGGTTGAA CTGCTGATCT TCGGATCTAT GTCGGGTGCG
1051 GAGAAAGAGG TAATGAAATG GCATCCGGAT CTGCATCGCA GGATGCTGCT
1101 GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCA TTATTGAAGC
1151 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
1201 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
1251 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG
1301 CGTATCACGA GGCCCTTTCG TCTTCAAGAA TTCTCATGTT TGACAGCTTA
1351 TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA TTGCTAACGC
1401 AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
1451 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC
1501 CGGGCCTCTT GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT
1551 GGCGTGCTGC TAGCGCTATA TGCGTTGATG CAATTTCTAT GCGCACCCGT
1601 TCTCGGAGCA CTGTCCGACC GCTTTGGCCG CCGCCCAGTC CTGCTCGCTT
1651 CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC CACACCCGTC
1701 CTGTGGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC
1751 AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC
1801 GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG
1851 GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC
1901 ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT
1951 TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGACCGAT GCCCTTGAGA
2001 GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT
2051 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC
2101 CGGCAGCGCT CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG
2151 ACGATGATCG GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC
2201 TCAAGCCTTC GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG
2251 CCATTATCGC CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG
2301 TTCGCGACGC GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC
2351 CGGCGGCATC GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG
2401 ATGACGACCA TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC
2451 CTAACTTCGA TCATTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC
2501 GGCGAGCACA TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC
2551 TTGTCTGCCT CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG
2601 ACCTGAATGG AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA
2651 ATTGGAGCCA ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC
2701 TTGGCAGAAC ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC
2751 GCATCTCGGG CAGCGTTGGG TCCTGGGCTG GCATTGACCC TGAGTGATTT
2801 TTCTCTGGTC CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT
2851 TCCAGTAACC GGGCATGTTC ATCATCAGTA ACCCGTATCG TGAGCATCCT
2901 CTCTCGTTTC ATCGGTATCA TTACCCCCAT GAACAGAAAT CCCCCTTACA
2951 CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA CATGGCCCGC
3001 TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA
3051 CGCGGATGAA CAGGCAGAGC TCTTACTGTC ATGCCATCCG TATGTGGGCG
3101 GACAATAAAG TCTTAAACTG AACAAAATAG ATCTAAACTA TGACAATAAA
3151 GTCTTAAACT AGACAGAATA GTTGTAAACT GAAATCAGTC CAGTTATGCT
3201 GTGAAAAAGC ATACTGGACT TTTGTTATGG CTAAAGCAAA CTCTTCATTT
3251 TCTGAAGTGC AAATTGCCCG TCGTATTAAA GAGGGGCGTG GGGTCGACGC
3301 GGCCGCTAAC TATAACGGTC CTAAGGTAGC GAGTTTAAAC GATATCGGAT
3351 CCGGCCGCCG CTGAGGTCTG CCTCGTGAAG AAGGTGTTGC TGACTCATAC
3401 CAGGCCTGAA TCGCCCCATC ATCCAGCCAG AAAGTGAGGG AGCCACGGTT
3451 GATGAGAGCT TTGTTGTAGG TGGACCAGTT GGTGATTTTG AACTTTTGCT
3501 TTGCCACGGA ACGGTCTGCG TTGTCGGGAA GATGCGTGAT CTGATCCTTC
3551 AACTCAGCAA GAGTTCGATT TATTCAACAA AGCCGCCGTC CCGTCAAGTC
3601 AGCGTAATGC TCTGCCAGTG TTACAACCAA TTAACCAATT CTGATTAGAA
3651 AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT
3701 CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA
3751 CCGAGGCAGT TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC
3801 GACTCGTCCA ACATCAATAC AACCTATTAA TTTCCCCTCG TCAAAAATAA
3851 GGTTATCAAG TGAGAAATCA CCATGAGTGA CGACTGAATC CGGTGAGAAT
3901 GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG GCCAGCCATT
3951 ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG
4001 ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA
4051 CAAACAGGAA TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC
4101 AATATTTTCA CCTGAATCAG GATATTCTTC TAATACCTGG AATGCTGTTT
4151 TCCCGGGGAT CGCAGTGGTG AGTAACCATG CATCATCAGG AGTACGGATA
4201 AAATGCTTGA TGGTCGGAAG AGGCATAAAT TCCGTCAGCC AGTTTAGTCT
4251 GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACCTTTG CCATGTTTCA
4301 GAAACAACTC TGGCGCATCG GGCTTCCCAT ACAATCGATA GATTGTCGCA
4351 CCTGATTGCC CGACATTATC GCGAGCCCAT TTATACCCAT ATAAATCAGC
4401 ATCCATGTTG GAATTTAATC GCGGCCTCGA GCAAGACGTT TCCCGTTGAA
4451 TATGGCTCAT AACACCCCTT GTATTACTGT TTATGTAAGC AGACAGTTTT
4501 ATTGTTCATG ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT
4551 TTGAGACACA ACGTGGCTTA CTAGGATCCG ATATCATTTA AATCTAGGGA
4601 TAACAGGGTA ATACTAGTGT CGACCAACCA GATAAGTGAA ATCTAGTTCC
4651 AAACTATTTT GTCATTTTTA ATTTTCGTAT TAGCTTACGA CGCTACACCC
4701 AGTTCCCATC TATTTTGTCA CTCTTCCCTA AATAATCCTT AAAAACTCCA
4751 TTTCCACCCC TCCCAGTTCC CAACTATTTT GTCCGCCCAC ACCGTAAGAT
4801 GCTTTTCTGT GACT
