Nonsense suppressor: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
Nonsense suppressors change the effects of nonsense mutations by altering how stop codons are read. The largest class of nonsense suppressors are mutations in [[:Category:tRNA genes]] that alter the codon-anticodon interaction to allow a stop codon to be read by that tRNA. This will only suppress the mutation if the amino acid inserted restores a functional protein product. | Nonsense suppressors change the effects of nonsense mutations by altering how stop codons are read. The largest class of nonsense suppressors are mutations in [[:Category:tRNA genes]] that alter the codon-anticodon interaction to allow a stop codon to be read by that tRNA. This will only suppress the mutation if the amino acid inserted restores a functional protein product. | ||
Nonsense suppression is defined genetically based on reversing an observable phenotype. Note that this does not mean full restoration of normal function. Nonsense suppression is often incomplete ("leaky") and [[wikipedia:Pleiotropy|pleiotropic]]. | |||
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.F46b4cf5d3f9bd--> | <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.F46b4cf5d3f9bd--> | ||
| Line 126: | Line 128: | ||
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.F46b4cf5d3f9bd--></protect> | <!--box uid=63ce1649aab8b3180235b0af7618f667.0.F46b4cf5d3f9bd--></protect> | ||
<small>This table is incomplete and may have inaccuracies in the synonym lists for amber vs ochre. Need to add the engineered suppressors from Kleina et al<ref name='PMID: 2193162'/> and Normanly et al<ref name='PMID: 2141650'/>. Others?</small> | <small>This table is incomplete and may have inaccuracies in the synonym lists for amber vs ochre. Need to add the engineered suppressors from Kleina et al<ref name='PMID: 2193162'/> and Normanly et al<ref name='PMID: 2141650'/>. Others?</small> | ||
== tRNA suppressors == | |||
== non-tRNA nonsense suppressors == | |||
== See Also == | == See Also == | ||
* [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/rev-sup/nonsense-suppressors.html http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/rev-sup/nonsense-suppressors.html] Stan Maloy's page on nonsense suppressors | * [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/rev-sup/nonsense-suppressors.html http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/rev-sup/nonsense-suppressors.html] Stan Maloy's page on nonsense suppressors | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 20:43, 4 August 2007
Nonsense suppressors change the effects of nonsense mutations by altering how stop codons are read. The largest class of nonsense suppressors are mutations in Category:tRNA genes that alter the codon-anticodon interaction to allow a stop codon to be read by that tRNA. This will only suppress the mutation if the amino acid inserted restores a functional protein product.
Nonsense suppression is defined genetically based on reversing an observable phenotype. Note that this does not mean full restoration of normal function. Nonsense suppression is often incomplete ("leaky") and pleiotropic.
| gene | synonyms | tRNA affected | aa inserted | codons recognized |
|---|---|---|---|---|
|
supD, supH, su1, Su-1, SuI, ftsM, su_1 |
Serine [1] |
Amber | ||
|
supE, Su2, Su_II |
Glutamine[2] |
Amber | ||
|
supF |
Tyrosine[2] |
Amber | ||
|
supG, supK, supL, Su(beta), su-5, |
Lysine |
Ochre | ||
|
supP, Su-6 |
Leucine |
Amber | ||
|
supC |
Tyrosine[2] |
Ochre | ||
|
supU, supV, su9 |
Tryptophan |
Opal | ||
|
supZ |
Tyrosine |
Amber | ||
|
supB |
Glutamine |
Ochre | ||
|
supL |
Lysine |
Ochre | ||
| edit table |
This table is incomplete and may have inaccuracies in the synonym lists for amber vs ochre. Need to add the engineered suppressors from Kleina et al[3] and Normanly et al[4]. Others?
tRNA suppressors
non-tRNA nonsense suppressors
See Also
- http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/rev-sup/nonsense-suppressors.html Stan Maloy's page on nonsense suppressors
References
- ↑ WEIGERT, MG & GAREN, A (1965) AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE. J. Mol. Biol. 12 448-55 PubMed
- ↑ 2.0 2.1 2.2 Weigert, MG et al. (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene. J. Mol. Biol. 14 522-7 PubMed
- ↑ Kleina, LG et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency. J. Mol. Biol. 213 705-17 PubMed
- ↑ Normanly, J et al. (1990) Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J. Mol. Biol. 213 719-26 PubMed