E. coli Genome Project at UW-Madison: Difference between revisions

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[http://www.genome.wisc.edu/functional/archivecloning.htm Archive Cloning] with each ORF cloned in a standard vector<BR>
[http://www.genome.wisc.edu/functional/archivecloning.htm Archive Cloning] with each ORF cloned in a standard vector<BR>
[http://www.genome.wisc.edu/functional/tnmutagenesis.htm Systematic Mutagenesis]: A strain collection consisting of a mutant in every gene<BR>
[http://www.genome.wisc.edu/functional/tnmutagenesis.htm Systematic Mutagenesis]: A strain collection consisting of a mutant in every gene<BR>
DNA Microarrays<BR>
[http://www.genome.wisc.edu/functional/microarray.htm DNA Microarray experiments] of wt and nutants inder different conditions<BR>
Phenotypic Microarrays<BR>
[http://www.genome.wisc.edu/functional/phenotypearray.htm Phenotypic analysis] of mutants<BR>
Protocols and Recipes<BR>
Protocols and Recipes<BR>
List of some essential genes in ''E. coli''
<BR>
<BR>
<BR>
<BR>

Revision as of 20:57, 21 August 2006

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E. coli Genome Project at UW-Madison
Who: proprietor
What: short description
Where: E. coli Genome Project at UW-Madison
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About E. coli Genome Project at UW-Madison

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Content

Genomic sequence data of various organisms and their annotation. There are several resources including:

  • Sequencing Projects

E.coli K12 MG1655
E.coli )157:H7
Uropathogenic E.coli
E.coli K1 (NMEC)
Shigella flexneri 2a
Salmonella typhi
Yersinia pestis

Plasmids
Other Enterobacterial Genome Projects See list

  • Functional Genomics

Archive Cloning with each ORF cloned in a standard vector
Systematic Mutagenesis: A strain collection consisting of a mutant in every gene
DNA Microarray experiments of wt and nutants inder different conditions
Phenotypic analysis of mutants
Protocols and Recipes
List of some essential genes in E. coli

  • BLAST & ASAP Database information

Using E. coli Genome Project at UW-Madison

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Discussion

References

M. Liu, T. Durfee, J. E. Cabrera, K. Zhao, D. J. Jin, and F. R. Blattner (2005) Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli. J Biol Chem 280(16): 15921-15927 PubMed

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E. coli Genome Project at UW-Madison

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