SM10(λpir): Difference between revisions
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| [https://bccm.belspo.be/ | | [https://bccm.belspo.be/page/genecorner-hosts-display-razor/359 LMBP 3889] | ||
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| References | | References | ||
Latest revision as of 11:32, 28 October 2024
| Name | SM10(λpir) |
| Synonyms | SM10(lambda pir) |
| Genotype | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km λpir |
| Phenotype | Kanamycin resistant |
| Sources | LMBP 3889 |
| References | Simon R, Priefer U, Puhler A (1983) A broad host range mobilization system for invivo genetic-engineering - transposon mutagenesis in gram-negative bacteria. Bio-Technol 1: 784–791 |
Notes
Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.
Useful for mobilizing mobRP4 plasmids.
The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.