SM10(λpir)

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Name SM10(λpir)
Synonyms SM10(lambda pir)
Genotype thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km λpir
Phenotype Kanamycin resistant
Sources LMBP 3889
References Simon R, Priefer U, Puhler A (1983) A broad host range mobilization system for invivo genetic-engineering - transposon mutagenesis in gram-negative bacteria. Bio-Technol 1: 784–791

Notes

Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.
Useful for mobilizing mobRP4 plasmids.
The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.